AMPDB_953 | Colicin-E7

PEPTIDE SUMMARY

Colicin-E7

1 General Description

AMPDB ID: AMPDB_953

Protein Names: Colicin-E7 (EC 3.1.-.-)

Protein Family: Colicin pyosin nuclease family

Gene Name: colE7 cea

Source Organism: Escherichia coli

Protein Length: 576 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNPWGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSGEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 61, 'R': 30, 'N': 35, 'D': 32, 'C': 0, 'Q': 22, 'E': 30, 'G': 63, 'H': 13, 'I': 17, 'L': 27, 'K': 52, 'M': 10, 'F': 19, 'P': 32, 'S': 46, 'T': 29, 'W': 8, 'Y': 5, 'V': 45

Frequencies of Amino Acids

'A': 10.59%, 'R': 5.21%, 'N': 6.08%, 'D': 5.56%, 'C': 0%, 'Q': 3.82%, 'E': 5.21%, 'G': 10.94%, 'H': 2.26%, 'I': 2.95%, 'L': 4.69%, 'K': 9.03%, 'M': 1.74%, 'F': 3.3%, 'P': 5.56%, 'S': 7.99%, 'T': 5.03%, 'W': 1.39%, 'Y': 0.87%, 'V': 7.81%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

282

Hydrophilic Amino Acid(s) Count

294

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	61349.6 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	63.038	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	38.32	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.682	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.858	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	10.423	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	21.228	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.21, 0.306, 0.222 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.056	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	51450, 51450	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Endonuclease, Hydrolase, Nuclease

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Soong BW, Lu FM, Chak KF, et al. Characterization of the cea gene of the ColE7 plasmid. Mol Gen Genet. 1992;233(1-2):177-83. Published 1992 May. doi:10.1007/BF00587577

PMID: 1603061

Citation 2: Ko TP, Liao CC, Ku WY, et al. The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein. Structure. 1999;7(1):91-102. Published 1999 Jan 15. doi:10.1016/s0969-2126(99)80012-4

PMID: 10368275

5.2 Protein Sequence Databases

UniProt: Q47112

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: Q47112

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M62409	GenBank \|\| EMBL
2.	X63620	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_021530049.1, YP_009060493.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Q47112

MINT: Not found

DIP: DIP-16990N

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR024575, IPR037146, IPR024566, IPR044925, IPR003615, IPR016128, IPR036302

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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