AMPDB_86 | Wheatwin-1

1 General Description

AMPDB ID: AMPDB_86

Protein Names: Wheatwin-1 (Pathogenesis-related protein 4a) (Protein 0.14) (RNase) (EC 3.1.-.-)

Protein Family: Nil

Gene Name: PR4A

Source Organism: Triticum aestivum (Wheat)

Protein Length: 146 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MAARPMLVVALLCAAAAAATAQQATNVRATYHYYRPAQNNWDLGAPAVSAYCATWDASKPLSWRSKYGWTAFCGPAGAHGQASCGKCLQVTNPATGAQITARIVDQCANGGLDLDWDTVFTKIDTNGIGYQQGHLNVNYQFVDCRD

FASTA format

Counts of Amino Acids

'A': 26, 'R': 6, 'N': 8, 'D': 9, 'C': 7, 'Q': 10, 'E': 0, 'G': 12, 'H': 3, 'I': 4, 'L': 9, 'K': 4, 'M': 2, 'F': 3, 'P': 6, 'S': 5, 'T': 11, 'W': 5, 'Y': 7, 'V': 9

Frequencies of Amino Acids

'A': 17.81%, 'R': 4.11%, 'N': 5.48%, 'D': 6.16%, 'C': 4.79%, 'Q': 6.85%, 'E': 0%, 'G': 8.22%, 'H': 2.05%, 'I': 2.74%, 'L': 6.16%, 'K': 2.74%, 'M': 1.37%, 'F': 2.05%, 'P': 4.11%, 'S': 3.42%, 'T': 7.53%, 'W': 3.42%, 'Y': 4.79%, 'V': 6.16%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	15634.6 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	70.411	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	14.174	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.136	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.612	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	7.73	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	0.834	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.253, 0.212, 0.253 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.103	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	37930, 38305	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

B.cinerea, F.culmorum, F.graminearum

4.2 Antimicrobial Activity

Antimicrobial, Fungicide, Plant defense

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Caruso C, Bertini L, Tucci M, et al. Isolation and characterisation of wheat cDNA clones encoding PR4 proteins. DNA Seq. 1999;10(4-5):301-7. Published 1999. doi:10.3109/10425179909033956

PMID: 10727084

Citation 2: Caruso C, Caporale C, Poerio E, et al. The amino acid sequence of a protein from wheat kernel closely related to proteins involved in the mechanisms of plant defence. J Protein Chem. 1993;12(4):379-86. Published 1993 Aug. doi:10.1007/BF01025037

PMID: 8251057

Citation 3: Caporale C, Caruso C, Facchiano A, et al. Probing the modelled structure of wheatwin1 by controlled proteolysis and sequence analysis of unfractionated digestion mixtures. Proteins. 1999;36(2):192-204. Published 1999 Aug 1. doi:

PMID: 10398366

Citation 4: Caruso C, Caporale C, Chilosi G, et al. Structural and antifungal properties of a pathogenesis-related protein from wheat kernel. J Protein Chem. 1996;15(1):35-44. Published 1996 Jan. doi:10.1007/BF01886809

PMID: 8838588

Citation 5: Caporale C, Di Berardino I, Leonardi L, et al. Wheat pathogenesis-related proteins of class 4 have ribonuclease activity. FEBS Lett. 2004;575(1-3):71-6. Published 2004 Sep 24. doi:10.1016/j.febslet.2004.07.091

PMID: 15388335

Citation 6: Bertini L, Caporale C, Testa M, et al. Structural basis of the antifungal activity of wheat PR4 proteins. FEBS Lett. 2009;583(17):2865-71. Published 2009 Sep 3. doi:10.1016/j.febslet.2009.07.045

PMID: 19647737

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: O64392

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AJ006098	GenBank \|\| EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: PTHR46351

PROSITE: PS00771, PS00772, PS51174

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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