AMPDB_821 | N-acetylmuramoyl-L-alanine amidase A

PEPTIDE SUMMARY

N-acetylmuramoyl-L-alanine amidase A

1 General Description

AMPDB ID: AMPDB_821

Protein Names: N-acetylmuramoyl-L-alanine amidase A (EC 3.5.1.28)

Protein Family: Nil

Gene Name: cwhA

Source Organism: Achromobacter lyticus

Protein Length: 177 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

AVDFGEAIWNPASSSNYSTASNQTSAVIMHTMEGSYAGSISWFQNPSAQVSAHYLIRKSDGQITQMVREYHQAWHAKNHNYYTIGIEHDGRAADAGNWSAAMVNASARLTKSICARRGVNCASAWKGPGYDTFHLVPDSVRVKGHGMLSGNENRYDPGKYFPWSNYYNLINGGGGNP

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 20, 'R': 8, 'N': 15, 'D': 7, 'C': 2, 'Q': 6, 'E': 5, 'G': 18, 'H': 8, 'I': 9, 'L': 5, 'K': 6, 'M': 5, 'F': 4, 'P': 7, 'S': 19, 'T': 7, 'W': 6, 'Y': 11, 'V': 9

Frequencies of Amino Acids

'A': 11.3%, 'R': 4.52%, 'N': 8.47%, 'D': 3.95%, 'C': 1.13%, 'Q': 3.39%, 'E': 2.82%, 'G': 10.17%, 'H': 4.52%, 'I': 5.08%, 'L': 2.82%, 'K': 3.39%, 'M': 2.82%, 'F': 2.26%, 'P': 3.95%, 'S': 10.73%, 'T': 3.95%, 'W': 3.39%, 'Y': 6.21%, 'V': 5.08%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	19395.4 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	56.893	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	26.979	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.563	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.635	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.627	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	2.602	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.249, 0.333, 0.198 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.119	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	49390, 49515	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.faecalis (Gram-positive), M.luteus (Gram-negative), P.acidilactici (Gram-positive), S.aureus (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Hydrolase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Li S, Norioka S, Sakiyama F, et al. Purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus. J Biochem. 2000;127(6):1033-9. Published 2000 Jun. doi:10.1093/oxfordjournals.jbchem.a022694

PMID: 10833271

5.2 Protein Sequence Databases

UniProt: P81717

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: P81717

5.4 Nucleotide Sequence Databases

No entries found in GenBank or EMBL

CCDS: Not found

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR036505, IPR002502

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: 3.5.1.28

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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