AMPDB_70 | Frenatin 2.3S

1 General Description

AMPDB ID: AMPDB_70

Protein Names: Frenatin 2.3S (F2.3S) [Cleaved into: Frenatin 2.1S (F2.1S)]

Protein Family: Frog skin active peptide (FSAP) family; Frenatin subfamily

Gene Name: Nil

Source Organism: Sphaenorhynchus lacteus (Orinoco lime treefrog) (Hyla lactea)

Protein Length: 71 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MAFLKKSLFLVLFLGLVSLSMGEREKREEEEEEEEENKEEEANEEGKGESEEKRGLVGTLLGHIGKAILGG

FASTA format

Counts of Amino Acids

'A': 3, 'R': 3, 'N': 2, 'D': 0, 'C': 0, 'Q': 0, 'E': 19, 'G': 10, 'H': 1, 'I': 2, 'L': 11, 'K': 7, 'M': 2, 'F': 3, 'P': 0, 'S': 4, 'T': 1, 'W': 0, 'Y': 0, 'V': 3

Frequencies of Amino Acids

'A': 4.23%, 'R': 4.23%, 'N': 2.82%, 'D': 0%, 'C': 0%, 'Q': 0%, 'E': 26.76%, 'G': 14.08%, 'H': 1.41%, 'I': 2.82%, 'L': 15.49%, 'K': 9.86%, 'M': 2.82%, 'F': 4.23%, 'P': 0%, 'S': 5.63%, 'T': 1.41%, 'W': 0%, 'Y': 0%, 'V': 4.23%

Missing Amino Acid(s)

C, D, P, Q, W, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

H, T

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	7907.9 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	87.887	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	84.785	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.625	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.498	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	4.255	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-8.879	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.268, 0.225, 0.493 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.042	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 0	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Gram Negative Bacteria and Gram Positive Bacteria

4.2 Antimicrobial Activity

Amphibian defense peptide, Antibiotic, Antimicrobial, Antiviral protein, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Anti-inflammatory, Cytotoxin, Hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: MuÃ±oz-Camargo C, MÃ©ndez MC, Salazar V, et al. Frog skin cultures secrete anti-yellow fever compounds. J Antibiot (Tokyo). 2016;69(11):783-790. Published 2016 Nov. doi:10.1038/ja.2016.16

PMID: 27049440

Citation 2: Conlon JM, Mechkarska M, Radosavljevic G, et al. A family of antimicrobial and immunomodulatory peptides related to the frenatins from skin secretions of the Orinoco lime frog Sphaenorhynchus lacteus (Hylidae). Peptides. 2014;56:132-40. Published 2014 Jun. doi:10.1016/j.peptides.2014.03.020

PMID: 24704757

Citation 3: Pantic JM, Radosavljevic GD, Jovanovic IP, et al. In vivo administration of the frog skin peptide frenatin 2.1S induces immunostimulatory phenotypes of mouse mononuclear cells. Peptides. 2015;71:269-75. Published 2015 Sep. doi:10.1016/j.peptides.2015.03.028

PMID: 25861850

Citation 4: Pantic JM, Jovanovic IP, Radosavljevic GD, et al. The frog skin host-defense peptide frenatin 2.1S enhances recruitment, activation and tumoricidal capacity of NK cells. Peptides. 2017;93:44-50. Published 2017 Jul. doi:10.1016/j.peptides.2017.05.006

PMID: 28526557

Citation 5: MuÃ±oz-Camargo C, Salazar VA, Barrero-Guevara L, et al. Unveiling the Multifaceted Mechanisms of Antibacterial Activity of Buforin II and Frenatin 2.3S Peptides from Skin Micro-Organs of the Orinoco Lime Treefrog (Sphaenorhynchus lacteus). Int J Mol Sci. 2018;19(8). Published 2018 Jul 25. doi:10.3390/ijms19082170

PMID: 30044391

Citation 6: Musale V, Guilhaudis L, Abdel-Wahab YHA, et al. Insulinotropic activity of the host-defense peptide frenatin 2D: Conformational, structure-function and mechanistic studies. Biochimie. 2019;156:12-21. Published 2019 Jan. doi:10.1016/j.biochi.2018.09.008

PMID: 30244134

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: L0L3V3

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	JX489597	GenBank \|\| EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR004275

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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