AMPDB_69 | Dermonecrotic toxin LgSicTox-alphaIC1
PEPTIDE SUMMARY
Dermonecrotic toxin LgSicTox-alphaIC1
1 General Description
AMPDB ID: AMPDB_69
Protein Names: Dermonecrotic toxin LgSicTox-alphaIC1 (EC 4.6.1.-) (Phospholipase D) (PLD) (Phospholipase D LgRec1) (Sphingomyelin phosphodiesterase D) (SMD) (SMase D) (Sphingomyelinase D) [Cleaved into: U1-sicaritoxin-Lg1a (U1-SCRTX-Lg1a) (Anionic antimicrobial peptide) (AAMP) (Lg-AMP1)]
Protein Family: Arthropod phospholipase D family; Class II subfamily
Gene Name: Nil
Protein Length: 280 AA
Protein Existence: Evidence at protein level
2 Protein Sequence & Composition
2.1 Sequence
ADNRRPIWVMGHMVNSLAQIDEFVGLGSNSIETDVSFDKQANPEYTYHGIPCDCGRACLHSTKFNDFLKGLRKVTTPGDSKYLEKLILVVFDLKTGSLYDNQAYDAGTKLAKNLLQHYWNNGNNGGRAYIILSIPNLNHYKLITGFKETLKNEGHEELLEKVGTDFSGNDDISDVQKTYNKAGVTGHVWQSDGITNCLLRGLTRVKAAVANRDSGSGIINKVYYWTVDKRQSTRDTLDANVDGIMTNYPDITVEILNEAAYKKKFRIATYEDNPWETFKG
FASTA format
2.2 Composition
Counts of Amino Acids
'A': 16, 'R': 11, 'N': 23, 'D': 22, 'C': 4, 'Q': 7, 'E': 13, 'G': 24, 'H': 7, 'I': 17, 'L': 24, 'K': 22, 'M': 3, 'F': 9, 'P': 7, 'S': 14, 'T': 21, 'W': 5, 'Y': 14, 'V': 17
Frequencies of Amino Acids
'A': 5.71%, 'R': 3.93%, 'N': 8.21%, 'D': 7.86%, 'C': 1.43%, 'Q': 2.5%, 'E': 4.64%, 'G': 8.57%, 'H': 2.5%, 'I': 6.07%, 'L': 8.57%, 'K': 7.86%, 'M': 1.07%, 'F': 3.21%, 'P': 2.5%, 'S': 5%, 'T': 7.5%, 'W': 1.79%, 'Y': 5%, 'V': 6.07%
Missing Amino Acid(s)
No Amino Acid(s) are missing in this protein
Most Occurring Amino Acid(s)
G, L
Less Occurring Amino Acid(s)
M
Hydrophobic Amino Acid(s) Count
122
Hydrophilic Amino Acid(s) Count
158
Basic Amino Acid(s) Count
35
Acidic Amino Acid(s) Count
40
Modified Amino Acid(s) Count
0
Modified Amino Acid(s) Frequencies
0
Computed by biopython (version 1.79) & proteinAnalysis (version 1)
3 Physicochemical Properties
Sl. No. Properties Values Reference
1. Molecular Mass 31447.3 Da Computed by ProtParam module (biopython 1.79)
2. Aliphatic Index 80.429 Computed by ProtParam module (biopython 1.79)
3. Instability Index (Half Life) 24.034 Computed by ProtParam module (biopython 1.79)
4. Hydrophobicity (GRAVY) -0.521 Computed by ProtParam module (biopython 1.79)
5. Hydrophobic Moment 0.826 Computed by ProtParam module (biopython 1.79)
6. Isoelectric Point 6.875 Computed by ProtParam module (biopython 1.79)
7. Charge (at pH 7) -1.599 Computed by ProtParam module (biopython 1.79)
8. Secondary Structure Fraction 0.307, 0.243, 0.2 [Helix, Turn, Sheet] Computed by ProtParam module (biopython 1.79)
9. Aromaticity 0.1 Computed by ProtParam module (biopython 1.79)
10. Molar Extinction Coefficient (cysteine|cystine) 48360, 48610 Computed by ProtParam module (biopython 1.79)
4 Activity Details
4.1 Target Organism(s)
L.gaucho
4.2 Antimicrobial Activity
Antibiotic, Antimicrobial, Anti-candida
4.3 Enzymatic Activity
Lyase
4.4 Inhibitory Effect
Not found
4.5 Other Biological Activity
Cytolysis, Toxin, Cytotoxin, Hemolytic, Non-ribosomal
Activity data manually curated from Literature and UniProt
5 Database Cross-references
5.1 Literature Database
5.1.1 PubMed
Citation 1: Magalhães GS, Caporrino MC, Della-Casa MS, et al. Cloning, expression and characterization of a phospholipase D from Loxosceles gaucho venom gland. Biochimie. 2013;95(9):1773-83. Published 2013 Sep. doi:10.1016/j.biochi.2013.06.002
PMID: 23770445
Citation 2: Segura-Ramírez PJ, Silva Júnior PI, Silva Júnior PI. Loxosceles gaucho Spider Venom: An Untapped Source of Antimicrobial Agents. Toxins (Basel). 2018;10(12). Published 2018 Dec 6. doi:10.3390/toxins10120522
PMID: 30563217
Citation 3: Lajoie DM, Roberts SA, Zobel-Thropp PA, et al. Variable Substrate Preference among Phospholipase D Toxins from Sicariid Spiders. J Biol Chem. 2015;290(17):10994-1007. Published 2015 Apr 24. doi:10.1074/jbc.M115.636951
PMID: 25752604
5.2 Protein Sequence Databases
UniProt: K9USW8
5.3 3D Structure Databases
No PDB Ids found
AlphaFoldDB: K9USW8
5.4 Nucleotide Sequence Databases
Sl. no. Accession(s) Access Link(s)
1. JX866729 GenBank || EMBL
CCDS: Not found
RefSeq: Not found
5.5 Protein-Protein Interaction Databases
STRING: Not found
IntAct: Not found
MINT: Not found
DIP: Not found
BioGRID: Not found
5.6 Ligand Databases
BindingDB: Not found
DrugBank: Not found
ChEMBL: Not found
5.7 Family & Domain Databases
InterPro: IPR017946
PANTHER: Not found
PROSITE: Not found
5.8 Genome Annotation Databases
Ensembl: Not found
KEGG: Not found
5.9 Phylogenomic Databases
GeneTree: Not found
5.10 Enzyme & Pathway Databases
BRENDA: 3.1.4.41
BioCyc: Not found
5.11 Protein-RNA Interaction Databases
RNAct: Not found




© 2023 B&BL, DoAS, IIIT-A, UP-211015, India