AMPDB_653 | Eumenine mastoparan-AF

1 General Description

AMPDB ID: AMPDB_653

Protein Names: Eumenine mastoparan-AF (EMP-AF) (Af-113)

Protein Family: MCD family; Mastoparan subfamily

Gene Name: Nil

Source Organism: Anterhynchium flavomarginatum micado (Solitary wasp)

Protein Length: 14 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

INLLKIAKGIIKSL

FASTA format

Counts of Amino Acids

'A': 1, 'R': 0, 'N': 1, 'D': 0, 'C': 0, 'Q': 0, 'E': 0, 'G': 1, 'H': 0, 'I': 4, 'L': 3, 'K': 3, 'M': 0, 'F': 0, 'P': 0, 'S': 1, 'T': 0, 'W': 0, 'Y': 0, 'V': 0

Frequencies of Amino Acids

'A': 7.14%, 'R': 0%, 'N': 7.14%, 'D': 0%, 'C': 0%, 'Q': 0%, 'E': 0%, 'G': 7.14%, 'H': 0%, 'I': 28.57%, 'L': 21.43%, 'K': 21.43%, 'M': 0%, 'F': 0%, 'P': 0%, 'S': 7.14%, 'T': 0%, 'W': 0%, 'Y': 0%, 'V': 0%

Missing Amino Acid(s)

C, D, E, F, H, M, P, Q, R, T, V, W, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

A, G, N, S

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	1523.97 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	202.143	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	-21.036	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	1.057	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.691	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	11.103	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	2.997	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.5, 0.214, 0.286 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 0	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

B.subtilis (Gram-positive), E.coli (Gram-negative), P.aeruginosa (Gram-negative), S.aureus (Gram-positive), S.epidermidis (Gram-positive), S.saprophyticus (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Cytolysis, Toxin, Hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Hisada M, Konno K, Itagaki Y, et al. Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom. Rapid Commun Mass Spectrom. 2000;14(19):1828-34. Published 2000. doi:10.1002/1097-0231(20001015)14:19<1828::AID-RCM101>3.0.CO;2-G

PMID: 11006592

Citation 2: Konno K, Hisada M, Naoki H, et al. Structure and biological activities of eumenine mastoparan-AF (EMP-AF), a new mast cell degranulating peptide in the venom of the solitary wasp (Anterhynchium flavomarginatum micado). Toxicon. 2000;38(11):1505-15. Published 2000 Nov. doi:10.1016/s0041-0101(00)00083-0

PMID: 10775751

Citation 3: dos Santos Cabrera MP, de Souza BM, Fontana R, et al. Conformation and lytic activity of eumenine mastoparan: a new antimicrobial peptide from wasp venom. J Pept Res. 2004;64(3):95-103. Published 2004 Sep. doi:10.1111/j.1399-3011.2004.00173.x

PMID: 15317499

Citation 4: Konno K, Kazuma K, Nihei K, et al. Peptide Toxins in Solitary Wasp Venoms. Toxins (Basel). 2016;8(4):114. Published 2016 Apr 18. doi:10.3390/toxins8040114

PMID: 27096870

Citation 5: Canduri F, Delatorre P, Fadel V, et al. Crystallization and preliminary X-ray diffraction analysis of a eumenine mastoparan toxin: a new class of mast-cell degranulating peptide in the wasp venom. Acta Crystallogr D Biol Crystallogr. 2000;56(Pt 11):1434-6. Published 2000 Nov. doi:10.1107/s0907444900009719

PMID: 11053843

Citation 6: Delatorre P, Olivieri JR, Ruggiero Neto J, et al. Preliminary cryocrystallography analysis of an eumenine mastoparan toxin isolated from the venom of the wasp Anterhynchium flavomarginatum micado. Biochim Biophys Acta. 2001;1545(1-2):372-6. Published 2001 Feb 9. doi:10.1016/s0167-4838(00)00192-8

PMID: 11342062

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: P0C022

5.4 Nucleotide Sequence Databases

No entries found in GenBank or EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: Not found

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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