AMPDB_650 | Colicin-N

PEPTIDE SUMMARY

Colicin-N

1 General Description

AMPDB ID: AMPDB_650

Protein Names: Colicin-N

Protein Family: Channel forming colicin family

Gene Name: cna

Source Organism: Escherichia coli

Protein Length: 387 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWSWSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNSKPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNWKGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVAKEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANKIGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGAVLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 29, 'R': 12, 'N': 43, 'D': 17, 'C': 0, 'Q': 3, 'E': 18, 'G': 43, 'H': 7, 'I': 27, 'L': 24, 'K': 39, 'M': 7, 'F': 12, 'P': 12, 'S': 37, 'T': 11, 'W': 6, 'Y': 12, 'V': 28

Frequencies of Amino Acids

'A': 7.49%, 'R': 3.1%, 'N': 11.11%, 'D': 4.39%, 'C': 0%, 'Q': 0.78%, 'E': 4.65%, 'G': 11.11%, 'H': 1.81%, 'I': 6.98%, 'L': 6.2%, 'K': 10.08%, 'M': 1.81%, 'F': 3.1%, 'P': 3.1%, 'S': 9.56%, 'T': 2.84%, 'W': 1.55%, 'Y': 3.1%, 'V': 7.24%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

G, N

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

188

Hydrophilic Amino Acid(s) Count

199

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	41743.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	79.871	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	17.923	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.458	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.757	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	10.221	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	16.652	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.282, 0.349, 0.202 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.078	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	50880, 50880	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Cell membrane, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Pugsley AP, Pugsley AP. Nucleotide sequencing of the structural gene for colicin N reveals homology between the catalytic, C-terminal domains of colicins A and N. Mol Microbiol. 1987;1(3):317-25. Published 1987 Nov. doi:10.1111/j.1365-2958.1987.tb01938.x

PMID: 2834623

Citation 2: Pugsley AP, Pugsley AP. The immunity and lysis genes of ColN plasmid pCHAP4. Mol Gen Genet. 1988;211(2):335-41. Published 1988 Feb. doi:10.1007/BF00330613

PMID: 3280946

Citation 3: Vetter IR, Parker MW, Tucker AD, et al. Crystal structure of a colicin N fragment suggests a model for toxicity. Structure. 1998;6(7):863-74. Published 1998 Jul 15. doi:10.1016/s0969-2126(98)00088-4

PMID: 9687368

5.2 Protein Sequence Databases

UniProt: P08083

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P08083

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	Y00533	GenBank \|\| EMBL
2.	X06933	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_032488335.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: P08083

MINT: Not found

DIP: DIP-29645N

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR000293, IPR038283

PANTHER: Not found

PROSITE: PS00276

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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