AMPDB_636 | Colicin-E3

1 General Description

AMPDB ID: AMPDB_636

Protein Names: Colicin-E3 (EC 3.1.-.-) (Colicin-E3 A chain) (Ribonuclease)

Protein Family: Cloacin colicin family

Gene Name: ceaC

Source Organism: Escherichia coli

Protein Length: 551 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRSAENNLNDEKNKPRKGFKDYGHDYHPAPKTENIKGLGDLKPGIPKTPKQNGGGKRKRWTGDKGRKIYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKYL

FASTA format

Counts of Amino Acids

'A': 58, 'R': 27, 'N': 38, 'D': 38, 'C': 0, 'Q': 22, 'E': 24, 'G': 71, 'H': 12, 'I': 18, 'L': 27, 'K': 41, 'M': 9, 'F': 12, 'P': 33, 'S': 44, 'T': 23, 'W': 8, 'Y': 9, 'V': 37

Frequencies of Amino Acids

'A': 10.53%, 'R': 4.9%, 'N': 6.9%, 'D': 6.9%, 'C': 0%, 'Q': 3.99%, 'E': 4.36%, 'G': 12.89%, 'H': 2.18%, 'I': 3.27%, 'L': 4.9%, 'K': 7.44%, 'M': 1.63%, 'F': 2.18%, 'P': 5.99%, 'S': 7.99%, 'T': 4.17%, 'W': 1.45%, 'Y': 1.63%, 'V': 6.72%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

273

Hydrophilic Amino Acid(s) Count

278

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	57963.1 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	61.851	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	36.799	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.733	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.729	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.648	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	7.129	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.201, 0.338, 0.214 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.053	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	57410, 57410	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Endonuclease, Hydrolase, Nuclease

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Masaki H, Ohta T, Ohta T. Colicin E3 and its immunity genes. J Mol Biol. 1985;182(2):217-27. Published 1985 Mar 20. doi:10.1016/0022-2836(85)90340-7

PMID: 3889348

Citation 2: Lau PC, Rowsome RW, Zuker M, et al. Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3. Nucleic Acids Res. 1984;12(22):8733-45. Published 1984 Nov 26. doi:10.1093/nar/12.22.8733

PMID: 6095211

Citation 3: Mock M, Miyada CG, Gunsalus RP, et al. Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin. Nucleic Acids Res. 1983;11(11):3547-57. Published 1983 Jun 11. doi:10.1093/nar/11.11.3547

PMID: 6344012

Citation 4: Masaki H, Ohta T, Ohta T. A plasmid region encoding the active fragment and the inhibitor protein of colicin E3--CA38. FEBS Lett. 1982;149(1):129-32. Published 1982 Nov 22. doi:10.1016/0014-5793(82)81087-9

PMID: 6295812

Citation 5: Suzuki K, Imahori K, Imahori K. Amino acid sequence of an active fragment (T2A) of colicin E3. J Biochem. 1978;84(5):1031-9. Published 1978 Nov. doi:10.1093/oxfordjournals.jbchem.a132217

PMID: 730747

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P00646

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	J01574	GenBank \|\| EMBL
2.	X02397	GenBank \|\| EMBL
3.	X01162	GenBank \|\| EMBL

RefSeq: WP_000012964.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: DB04039, DB04147

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

Download