AMPDB_5630 | Colicin E3

PEPTIDE SUMMARY

Colicin E3

1 General Description

AMPDB ID: AMPDB_5630

Protein Names: Colicin E3

Protein Family: Nil

Gene Name: BR158_005176 DEO15_26275

Source Organism: Escherichia coli

Protein Length: 551 AA

Protein Existence: Predicted

2 Protein Sequence & Composition

2.1 Sequence

MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRSAENNLNDEKNKPRKGFKDYGHDYHPAPKTENIKGLGDLKPGIPKTPKQNGGGKRKRWTGDKGRKIYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKYL

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 58, 'R': 27, 'N': 38, 'D': 38, 'C': 0, 'Q': 22, 'E': 24, 'G': 71, 'H': 12, 'I': 18, 'L': 27, 'K': 41, 'M': 9, 'F': 12, 'P': 33, 'S': 44, 'T': 23, 'W': 8, 'Y': 9, 'V': 37

Frequencies of Amino Acids

'A': 10.53%, 'R': 4.9%, 'N': 6.9%, 'D': 6.9%, 'C': 0%, 'Q': 3.99%, 'E': 4.36%, 'G': 12.89%, 'H': 2.18%, 'I': 3.27%, 'L': 4.9%, 'K': 7.44%, 'M': 1.63%, 'F': 2.18%, 'P': 5.99%, 'S': 7.99%, 'T': 4.17%, 'W': 1.45%, 'Y': 1.63%, 'V': 6.72%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

273

Hydrophilic Amino Acid(s) Count

278

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	57963.1 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	61.851	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	36.799	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.733	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.729	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.648	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	7.129	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.201, 0.338, 0.214 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.053	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	57410, 57410	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Endonuclease, Hydrolase, Nuclease

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Morales M, Attai H, Troy K, et al. Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38. Plasmid. 2015;77:7-16. Published 2015 Jan. doi:10.1016/j.plasmid.2014.11.001

PMID: 25450765

Citation 2: Montero DA, Canto FD, Velasco J, et al. Cumulative acquisition of pathogenicity islands has shaped virulence potential and contributed to the emergence of LEE-negative Shiga toxin-producing Escherichia coli strains. Emerg Microbes Infect. 2019;8(1):486-502. Published 2019. doi:10.1080/22221751.2019.1595985

PMID: 30924410

5.2 Protein Sequence Databases

UniProt: A0A0B5ECT4

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: A0A0B5ECT4

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	KM287568	GenBank \|\| EMBL
2.	AASSAH010000104	GenBank \|\| EMBL
3.	QERO01000045	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_000012964.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR024575, IPR036725, IPR009105, IPR024566, IPR016128, IPR036302

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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