AMPDB_548 | Rugosin-LK1

PEPTIDE SUMMARY

Rugosin-LK1

1 General Description

AMPDB ID: AMPDB_548

Protein Names: Rugosin-LK1

Protein Family: Frog skin active peptide (FSAP) family; Brevinin subfamily

Gene Name: Nil

Source Organism: Limnonectes kuhlii (Kuhl`s Creek frog) (Rana kuhlii)

Protein Length: 75 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MFTMKKSLLFLFFLGTISLSFCEEERSADEDDEGEMTEEEKRSIRDKIKTMAIDLAKSAGTGVLKTLICKLDKSC

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 4, 'R': 3, 'N': 0, 'D': 6, 'C': 3, 'Q': 0, 'E': 9, 'G': 4, 'H': 0, 'I': 5, 'L': 9, 'K': 9, 'M': 4, 'F': 5, 'P': 0, 'S': 7, 'T': 6, 'W': 0, 'Y': 0, 'V': 1

Frequencies of Amino Acids

'A': 5.33%, 'R': 4%, 'N': 0%, 'D': 8%, 'C': 4%, 'Q': 0%, 'E': 12%, 'G': 5.33%, 'H': 0%, 'I': 6.67%, 'L': 12%, 'K': 12%, 'M': 5.33%, 'F': 6.67%, 'P': 0%, 'S': 9.33%, 'T': 8%, 'W': 0%, 'Y': 0%, 'V': 1.33%

Missing Amino Acid(s)

H, N, P, Q, W, Y

Most Occurring Amino Acid(s)

E, K, L

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	8474.85 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	82	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	41.736	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.204	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.67	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	4.644	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-3.172	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.267, 0.147, 0.347 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.067	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 125	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

B.subtilis (Gram-positive), C.albicans, E.coli (Gram-negative), P.aeruginosa (Gram-negative), S.aureus (Gram-positive)

4.2 Antimicrobial Activity

Amphibian defense peptide, Antibiotic, Antimicrobial, Fungicide, Anti-candida, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Wang G, Wang Y, Ma D, et al. Five novel antimicrobial peptides from the Kuhl's wart frog skin secretions, Limnonectes kuhlii. Mol Biol Rep. 2013;40(2):1097-102. Published 2013 Feb. doi:10.1007/s11033-012-2152-4

PMID: 23054029

5.2 Protein Sequence Databases

UniProt: C3RT23

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: C3RT23

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	EU346906	GenBank \|\| EMBL

CCDS: Not found

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR012521, IPR004275

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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