AMPDB_483 | Alpha-defensin 1

1 General Description

AMPDB ID: AMPDB_483

Protein Names: Alpha-defensin 1

Protein Family: Alpha-defensin family

Gene Name: DEFA1

Source Organism: Equus caballus (Horse)

Protein Length: 98 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MRTLTLLTALLLLALQVQTQSLEETADQVPAQDQPGAEAQDITISFAGDERSAREASKSLIGTASCTCRRAWICRWGERHSGKCIDQKGSTYRLCCRR

FASTA format

Counts of Amino Acids

'A': 11, 'R': 10, 'N': 0, 'D': 5, 'C': 6, 'Q': 8, 'E': 6, 'G': 6, 'H': 1, 'I': 5, 'L': 11, 'K': 3, 'M': 1, 'F': 1, 'P': 2, 'S': 8, 'T': 9, 'W': 2, 'Y': 1, 'V': 2

Frequencies of Amino Acids

'A': 11.22%, 'R': 10.2%, 'N': 0%, 'D': 5.1%, 'C': 6.12%, 'Q': 8.16%, 'E': 6.12%, 'G': 6.12%, 'H': 1.02%, 'I': 5.1%, 'L': 11.22%, 'K': 3.06%, 'M': 1.02%, 'F': 1.02%, 'P': 2.04%, 'S': 8.16%, 'T': 9.18%, 'W': 2.04%, 'Y': 1.02%, 'V': 2.04%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

A, L

Less Occurring Amino Acid(s)

F, H, M, Y

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	10843.3 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	80.816	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	50.536	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.363	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.747	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.118	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	1.728	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.224, 0.163, 0.296 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.041	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	12490, 12865	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

P.multocida (Gram-negative), R.equi, S.choleraesuis, S.dysgalactiae (Gram-positive), S.equi (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Defensin, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Bruhn O, Regenhard P, Michalek M, et al. A novel horse alpha-defensin: gene transcription, recombinant expression and characterization of the structure and function. Biochem J. 2007;407(2):267-76. Published 2007 Oct 15. doi:10.1042/BJ20070747

PMID: 17620056

Citation 2: Bruhn O, Cauchard J, Schlusselhuber M, et al. Antimicrobial properties of the equine alpha-defensin DEFA1 against bacterial horse pathogens. Vet Immunol Immunopathol. 2009;130(1-2):102-6. Published 2009 Jul 15. doi:10.1016/j.vetimm.2009.01.005

PMID: 19211153

Citation 3: Schlusselhuber M, Jung S, Bruhn O, et al. In vitro potential of equine DEFA1 and eCATH1 as alternative antimicrobial drugs in rhodococcosis treatment. Antimicrob Agents Chemother. 2012;56(4):1749-55. Published 2012 Apr. doi:10.1128/AAC.05797-11

PMID: 22232283

Citation 4: Michalek M, Jung S, Shomali MR, et al. Solution structure and functional studies of the highly potent equine antimicrobial peptide DEFA1. Biochem Biophys Res Commun. 2015;459(4):668-72. Published 2015 Apr 17. doi:10.1016/j.bbrc.2015.02.168

PMID: 25769951

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: A6YB85

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	EF379126	GenBank \|\| EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR016327, IPR006081, IPR002366

PANTHER: PTHR11876

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: ENSGT00940000153268

5.9 Phylogenomic Databases

GeneTree: ENSGT00940000153268

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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