AMPDB_481 | Probable N-acetylmuramidase

PEPTIDE SUMMARY

Probable N-acetylmuramidase

1 General Description

AMPDB ID: AMPDB_481

Protein Names: Probable N-acetylmuramidase (EC 3.2.1.17) (Autolysin) (Lysozyme) (Peptidoglycan hydrolase)

Protein Family: Glycosyl hydrolase 73 family

Gene Name: acmA llmg_0280

Source Organism: Lactococcus lactis subsp. cremoris (strain MG1363)

Protein Length: 437 AA

Protein Existence: Inferred from homology

2 Protein Sequence & Composition

2.1 Sequence

MPVSRVKVKNRHLKKKTKKPLAFYKPATKFAGAVLIAGTLTTTHELLLQQTSPMVQAATNSSEVFIESIAASAKPVADANGLYPSVMIAQAILESNWGSSQLSRAPYYNLFGIQGTYQGKSVVFKTQEYLNGKWVTKDMPFRVYPSFNQSFQDNAYVLKTTNFGNGPYYAKAWRANAATYQDATAALTGRYATDPSYGASLNRIISQYNLTRFDGASSAGNTNSGGSTTTITNNNSGTNSSSTTYTVKSGDTLWGISQRYGISVAQIQSANNLKSTIIYIGQKLVLTGSASSTNSGGSNNSASTTPTTSVTPAKPTSQTTVKVKSGDTLWALSVKYKTSIAQLKSWNHLSSDTIYIGQNLIVSQSAAASNPSTGSGSTATNNSNSTSSNSNASIHKVVKGDTLWGLSQKSGSPIASIKAWNHLSSDTILIGQYLRIK

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 42, 'R': 10, 'N': 33, 'D': 11, 'C': 0, 'Q': 22, 'E': 5, 'G': 32, 'H': 5, 'I': 25, 'L': 30, 'K': 30, 'M': 4, 'F': 10, 'P': 16, 'S': 63, 'T': 48, 'W': 8, 'Y': 20, 'V': 23

Frequencies of Amino Acids

'A': 9.61%, 'R': 2.29%, 'N': 7.55%, 'D': 2.52%, 'C': 0%, 'Q': 5.03%, 'E': 1.14%, 'G': 7.32%, 'H': 1.14%, 'I': 5.72%, 'L': 6.86%, 'K': 6.86%, 'M': 0.92%, 'F': 2.29%, 'P': 3.66%, 'S': 14.42%, 'T': 10.98%, 'W': 1.83%, 'Y': 4.58%, 'V': 5.26%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

190

Hydrophilic Amino Acid(s) Count

247

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	46564.1 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	73.959	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	25.604	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.338	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.608	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	10.444	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	24.44	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.265, 0.33, 0.185 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.087	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	73800, 73800	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

L.lactis (Gram-positive)

4.2 Antimicrobial Activity

Antimicrobial, Bacteriolytic enzyme, Anti-gram-Positive

4.3 Enzymatic Activity

Glycosidase, Hydrolase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Buist G, Kok J, Leenhouts KJ, et al. Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation. J Bacteriol. 1995;177(6):1554-63. Published 1995 Mar. doi:10.1128/jb.177.6.1554-1563.1995

PMID: 7883712

Citation 2: Wegmann U, O'Connell-Motherway M, Zomer A, et al. Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363. J Bacteriol. 2007;189(8):3256-70. Published 2007 Apr. doi:10.1128/JB.01768-06

PMID: 17307855

5.2 Protein Sequence Databases

UniProt: A2RHZ5

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: A2RHZ5

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	U17696	GenBank \|\| EMBL
2.	AM406671	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_011834353.1

5.5 Protein-Protein Interaction Databases

STRING: 416870.llmg_0280

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR018392, IPR036779, IPR002901

PANTHER: Not found

PROSITE: PS51782

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: llm:llmg_0280

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: LLAC416870:LLMG_RS01460-MON

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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