AMPDB_4400 | Colicin-5

PEPTIDE SUMMARY

Colicin-5

1 General Description

AMPDB ID: AMPDB_4400

Protein Names: Colicin-5

Protein Family: Channel forming colicin family

Gene Name: cfa

Source Organism: Escherichia coli

Protein Length: 490 AA

Protein Existence: Inferred from homology

2 Protein Sequence & Composition

2.1 Sequence

MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGGGGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLKDIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAERQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAESDVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALNSAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQDLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVPLGFWGIAIVTGIVSSYIGDDELNKLNELLGI

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 75, 'R': 22, 'N': 20, 'D': 32, 'C': 0, 'Q': 22, 'E': 46, 'G': 26, 'H': 5, 'I': 21, 'L': 37, 'K': 46, 'M': 6, 'F': 13, 'P': 6, 'S': 38, 'T': 25, 'W': 4, 'Y': 9, 'V': 37

Frequencies of Amino Acids

'A': 15.31%, 'R': 4.49%, 'N': 4.08%, 'D': 6.53%, 'C': 0%, 'Q': 4.49%, 'E': 9.39%, 'G': 5.31%, 'H': 1.02%, 'I': 4.29%, 'L': 7.55%, 'K': 9.39%, 'M': 1.22%, 'F': 2.65%, 'P': 1.22%, 'S': 7.76%, 'T': 5.1%, 'W': 0.82%, 'Y': 1.84%, 'V': 7.55%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

225

Hydrophilic Amino Acid(s) Count

265

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	53137.6 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	83.367	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	23.042	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.458	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.805	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	5.174	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-9.473	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.247, 0.184, 0.335 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.053	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	35410, 35410	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Pilsl H, Braun V, Braun V. Evidence that the immunity protein inactivates colicin 5 immediately prior to the formation of the transmembrane channel. J Bacteriol. 1995;177(23):6966-72. Published 1995 Dec. doi:10.1128/jb.177.23.6966-6972.1995

PMID: 7592492

5.2 Protein Sequence Databases

UniProt: Q47500

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: Q47500

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	X87835	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_000362089.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Q47500

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR000293, IPR038283

PANTHER: Not found

PROSITE: PS00276

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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