AMPDB_4349 | Probable autolysin LytO

PEPTIDE SUMMARY

Probable autolysin LytO

1 General Description

AMPDB ID: AMPDB_4349

Protein Names: Probable autolysin LytO (EC 3.5.1.28)

Protein Family: N-acetylmuramoyl-L-alanine amidase 2 family

Gene Name: lytO SAOUHSC_02019

Source Organism: Staphylococcus aureus (strain NCTC 8325 PS 47)

Protein Length: 481 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEIS

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 36, 'R': 19, 'N': 28, 'D': 21, 'C': 6, 'Q': 25, 'E': 24, 'G': 46, 'H': 9, 'I': 22, 'L': 33, 'K': 31, 'M': 7, 'F': 20, 'P': 27, 'S': 26, 'T': 28, 'W': 17, 'Y': 25, 'V': 31

Frequencies of Amino Acids

'A': 7.48%, 'R': 3.95%, 'N': 5.82%, 'D': 4.37%, 'C': 1.25%, 'Q': 5.2%, 'E': 4.99%, 'G': 9.56%, 'H': 1.87%, 'I': 4.57%, 'L': 6.86%, 'K': 6.44%, 'M': 1.46%, 'F': 4.16%, 'P': 5.61%, 'S': 5.41%, 'T': 5.82%, 'W': 3.53%, 'Y': 5.2%, 'V': 6.44%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

239

Hydrophilic Amino Acid(s) Count

242

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	54030 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	70.769	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	38.478	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.466	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.777	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.642	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	5.466	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.308, 0.264, 0.208 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.129	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	130750, 131125	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

S.aureus (Gram-positive)

4.2 Antimicrobial Activity

Antimicrobial, Bacteriolytic enzyme, Anti-gram-Positive

4.3 Enzymatic Activity

Hydrolase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Osipovitch DC, Therrien S, Griswold KE, et al. Discovery of novel S. aureus autolysins and molecular engineering to enhance bacteriolytic activity. Appl Microbiol Biotechnol. 2015;99(15):6315-26. Published 2015 Aug. doi:10.1007/s00253-015-6443-2

PMID: 25690309

5.2 Protein Sequence Databases

UniProt: Q2FX77

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: Q2FX77

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	CP000253	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_001148145.1, YP_500516.1

5.5 Protein-Protein Interaction Databases

STRING: 1280.SAXN108_1909

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR036505, IPR002502, IPR007921, IPR038765, IPR003646

PANTHER: Not found

PROSITE: PS50911, PS51781

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: sao:SAOUHSC_02019

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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