AMPDB_383 | Guanylate-binding protein 7

1 General Description

AMPDB ID: AMPDB_383

Protein Names: Guanylate-binding protein 7 (EC 3.6.5.-) (GTP-binding protein 7) (GBP-7) (Guanine nucleotide-binding protein 7) (Guanylate-binding protein 4-like)

Protein Family: TRAFAC class dynamin-like GTPase superfamily; GB1 RHD3 GTPase family; GB1 subfamily

Gene Name: Gbp7 Gbp4l

Source Organism: Mus musculus (Mouse)

Protein Length: 638 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MASGPNMEAPVCLVENENEELRVNSKAINILERITQPVVVVAIVGLYRTGKSYLMNRLAGQNHGFNLGTTVRSETKGIWMWCVPHPSKPKFTLVLLDTEGLGDVEKGDPKNDSWIFALAVLLSSTFVYNSMSTINHQALEQLHYVTELTERIRAKSTSRSEEVDDSDEFVSFFPDFIWTVRDFVLELKLEGRVITADEYLENALKLIPGMSIKAQKANLPRECIRHFFPRRKCFVFDRPTKDKELLVHVEEMPEDQLDHSFQVQSKEFCSYIFSNSKAKTLKEGIVVNGNRLATLVTTYVDAINSGDVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHTACEKEAIAVFMEHSFKDENQQFQKNLVVTIEEKKEDFLRQNEAASLSHCQAELDKLSESLRESISRGVFSVPGGHRLYLEARKKVEQDYERVPRKGVKANHVLQSFLQSQISIEDSIMQSDKALTDGQKAMEAERAQKEAAEKEQELLRQKQKELQQVMEAQERSYKENVAQLHEKMETERKNILREQEVKLEHKLKIQKDMLNEGFKRKCEAMDLEISQLQKEIQLNKEKNSSLGAKILDGFGDVLISVVPGSGKYFGLGLKILSSQMNQTQNSDKVRKL

FASTA format

Counts of Amino Acids

'A': 40, 'R': 33, 'N': 30, 'D': 30, 'C': 9, 'Q': 40, 'E': 66, 'G': 28, 'H': 15, 'I': 30, 'L': 65, 'K': 53, 'M': 17, 'F': 25, 'P': 18, 'S': 46, 'T': 29, 'W': 4, 'Y': 12, 'V': 48

Frequencies of Amino Acids

'A': 6.27%, 'R': 5.17%, 'N': 4.7%, 'D': 4.7%, 'C': 1.41%, 'Q': 6.27%, 'E': 10.34%, 'G': 4.39%, 'H': 2.35%, 'I': 4.7%, 'L': 10.19%, 'K': 8.31%, 'M': 2.66%, 'F': 3.92%, 'P': 2.82%, 'S': 7.21%, 'T': 4.55%, 'W': 0.63%, 'Y': 1.88%, 'V': 7.52%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

275

Hydrophilic Amino Acid(s) Count

363

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

101

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	72720.9 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	86.16	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	40.533	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.502	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.765	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	6.265	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-9.092	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.288, 0.191, 0.295 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.064	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	39880, 40380	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Listeria monocytogenes (Gram-positive), Mycobacterium bovis (Gram-positive), T.gondii, Toxoplasma gondii

4.2 Antimicrobial Activity

Antimicrobial, Anti-listeria, Anti-parasitic, Anti-protozoal, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Signal peptide, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Okazaki N, Kikuno R, Ohara R, et al. Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. DNA Res. 2004;11(2):127-35. Published 2004 Apr 30. doi:10.1093/dnares/11.2.127

PMID: 15449545

Citation 2: Carninci P, Kasukawa T, Katayama S, et al. The transcriptional landscape of the mammalian genome. Science. 2005;309(5740):1559-63. Published 2005 Sep 2. doi:10.1126/science.1112014

PMID: 16141072

Citation 3: Church DM, Goodstadt L, Hillier LW, et al. Lineage-specific biology revealed by a finished genome assembly of the mouse. PLoS Biol. 2009;7(5):e1000112. Published 2009 May 5. doi:10.1371/journal.pbio.1000112

PMID: 19468303

Citation 4: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 5: Degrandi D, Konermann C, Beuter-Gunia C, et al. Extensive characterization of IFN-induced GTPases mGBP1 to mGBP10 involved in host defense. J Immunol. 2007;179(11):7729-40. Published 2007 Dec 1. doi:10.4049/jimmunol.179.11.7729

PMID: 18025219

Citation 6: Huttlin EL, Jedrychowski MP, Elias JE, et al. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010;143(7):1174-89. Published 2010 Dec 23. doi:10.1016/j.cell.2010.12.001

PMID: 21183079

Citation 7: Kim BH, Shenoy AR, Kumar P, et al. A family of IFN-Î³-inducible 65-kD GTPases protects against bacterial infection. Science. 2011;332(6030):717-21. Published 2011 May 6. doi:10.1126/science.1201711

PMID: 21551061

Citation 8: Legewie L, Loschwitz J, Steffens N, et al. Biochemical and structural characterization of murine GBP7, a guanylate binding protein with an elongated C-terminal tail. Biochem J. 2019;476(21):3161-3182. Published 2019 Nov 15. doi:10.1042/BCJ20190364

PMID: 31689351

Citation 9: Steffens N, Beuter-Gunia C, Kravets E, et al. Essential Role of mGBP7 for Survival of Toxoplasma gondii Infection. mBio. 2020;11(1). Published 2020 Jan 21. doi:10.1128/mBio.02993-19

PMID: 31964735

Citation 10: Feng M, Zhang Q, Wu W, et al. Inducible Guanylate-Binding Protein 7 Facilitates Influenza A Virus Replication by Suppressing Innate Immunity via NF-ÎºB and JAK-STAT Signaling Pathways. J Virol. 2021;95(6). Published 2021 Feb 24. doi:10.1128/JVI.02038-20

PMID: 33408175

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: Q91Z40

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AK087751	GenBank \|\| EMBL
2.	AK131176	GenBank \|\| EMBL
3.	AK036666	GenBank \|\| EMBL
4.	GL456099	GenBank \|\| EMBL
5.	BC010229	GenBank \|\| EMBL
6.	BK005760	GenBank \|\| EMBL