AMPDB_3799 | Autolysin

PEPTIDE SUMMARY

Autolysin

1 General Description

AMPDB ID: AMPDB_3799

Protein Names: Autolysin (EC 3.2.1.-) (Beta-glycosidase) (Peptidoglycan hydrolase)

Protein Family: Glycosyl hydrolase 73 family

Gene Name: EF_0799

Source Organism: Enterococcus faecalis (strain ATCC 700802 V583)

Protein Length: 737 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MKKESMSRIERRKAQQRKKTPVQWKKSTTLFSSALIVSSVGTPVALLPVTAEATEEQPTNAEVAQAPTTETGLVETPTTETTPGTTEQPTTDSSTTTESTTESSKETPTTPSTEQPTADSTTPVESGTTDSSVAEITPVAPSATESEAAPAVTPDDEVKVPEARVASAQTFSALSPTQSPSEFIAELARCAQPIAQANDLYASVMMAQAIVESGWGASTLSKAPNYNLFGIKGSYNGQSVYMDTWEYLNGKWLVKKEPFRKYPSYMESFQDNAHVLKTTSFQAGVYYYAGAWKSNTSSYRDATAWLTGRYATDPSYNAKLNNVITAYNLTQYDTPSSGGNTGGGTVNPGTGGSNNQSGTNTYYTVKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFVGQKLIVKKGASGNTGGSGSGGSNNNQSGTNTYYTVKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFVGQKLIVKKGASGNTGGSNNGGSNNNQSGTNTYYTIKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFAGQKIIVKKGTSGNTGGSSNGGSNNNQSGTNTYYTIKSGDTLNKISAQFGVSVANLQAWNNISGSLIFAGQKIIVKKGANSGSTNTNKPTNNGGGATTSYTIKSGDTLNKISAQFGVSVANLRSWNGIKGDLIFAGQTIIVKKGASAGGNASSTNSASGKRHTVKSGDSLWGLSMQYGISIQKIKQLNGLSGDTIYIGQTLKVG

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 63, 'R': 14, 'N': 59, 'D': 22, 'C': 1, 'Q': 35, 'E': 26, 'G': 84, 'H': 2, 'I': 39, 'L': 38, 'K': 49, 'M': 7, 'F': 14, 'P': 27, 'S': 89, 'T': 84, 'W': 12, 'Y': 29, 'V': 43

Frequencies of Amino Acids

'A': 8.55%, 'R': 1.9%, 'N': 8.01%, 'D': 2.99%, 'C': 0.14%, 'Q': 4.75%, 'E': 3.53%, 'G': 11.4%, 'H': 0.27%, 'I': 5.29%, 'L': 5.16%, 'K': 6.65%, 'M': 0.95%, 'F': 1.9%, 'P': 3.66%, 'S': 12.08%, 'T': 11.4%, 'W': 1.63%, 'Y': 3.93%, 'V': 5.83%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

327

Hydrophilic Amino Acid(s) Count

410

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	77025 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	66.214	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	28.448	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.467	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.651	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.756	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	15.135	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.237, 0.351, 0.182 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.075	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	109210, 109210	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.faecalis (Gram-positive)

4.2 Antimicrobial Activity

Antimicrobial, Bacteriolytic enzyme, Anti-gram-Positive

4.3 Enzymatic Activity

Glycosidase, Hydrolase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: BÃ©liveau C, Potvin C, Trudel J, et al. Cloning, sequencing, and expression in Escherichia coli of a Streptococcus faecalis autolysin. J Bacteriol. 1991;173(18):5619-23. Published 1991 Sep. doi:10.1128/jb.173.18.5619-5623.1991

PMID: 1679432

Citation 2: Paulsen IT, Banerjei L, Myers GS, et al. Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 2003;299(5615):2071-4. Published 2003 Mar 28. doi:10.1126/science.1080613

PMID: 12663927

5.2 Protein Sequence Databases

UniProt: P37710

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P37710

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M58002	GenBank \|\| EMBL
2.	AE016830	GenBank \|\| EMBL

CCDS: Not found

RefSeq: NP_814543.1, WP_010706701.1

5.5 Protein-Protein Interaction Databases

STRING: 226185.EF_0799

IntAct: P37710

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR018392, IPR036779, IPR002901

PANTHER: Not found

PROSITE: PS51782

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: efa:EF0799

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

Download