AMPDB_3744 | Defensin-1

PEPTIDE SUMMARY

Defensin-1

1 General Description

AMPDB ID: AMPDB_3744

Protein Names: Defensin-1 (Royalisin)

Protein Family: Invertebrate defensin family; Type 1 subfamily

Gene Name: Nil

Source Organism: Apis mellifera (Honeybee)

Protein Length: 95 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MKIYFIVGLLFMAMVAIMAAPVEDEFEPLEHFENEERADRHRRVTCDLLSFKGQVNDSACAANCLSLGKAGGHCEKGVCICRKTSFKDLWDKRFG

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 9, 'R': 6, 'N': 3, 'D': 6, 'C': 6, 'Q': 1, 'E': 8, 'G': 7, 'H': 3, 'I': 4, 'L': 8, 'K': 7, 'M': 4, 'F': 7, 'P': 2, 'S': 4, 'T': 2, 'W': 1, 'Y': 1, 'V': 6

Frequencies of Amino Acids

'A': 9.47%, 'R': 6.32%, 'N': 3.16%, 'D': 6.32%, 'C': 6.32%, 'Q': 1.05%, 'E': 8.42%, 'G': 7.37%, 'H': 3.16%, 'I': 4.21%, 'L': 8.42%, 'K': 7.37%, 'M': 4.21%, 'F': 7.37%, 'P': 2.11%, 'S': 4.21%, 'T': 2.11%, 'W': 1.05%, 'Y': 1.05%, 'V': 6.32%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Q, W, Y

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	10717.4 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	77.053	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	35.038	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.081	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.526	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	6.743	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-1.087	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.284, 0.168, 0.305 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.095	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	6990, 7365	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

Gram Positive Bacteria

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Defensin, Anti-gram-Positive

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Klaudiny J, Albert S, BachanovÃ¡ K, et al. Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera. Insect Biochem Mol Biol. 2005;35(1):11-22. Published 2005 Jan. doi:10.1016/j.ibmb.2004.09.007

PMID: 15607651

Citation 2: Casteels-Josson K, Zhang W, Capaci T, et al. Acute transcriptional response of the honeybee peptide-antibiotics gene repertoire and required post-translational conversion of the precursor structures. J Biol Chem. 1994;269(46):28569-75. Published 1994 Nov 18. doi:

PMID: 7961803

Citation 3: Viljakainen L, Evans JD, Hasselmann M, et al. Rapid evolution of immune proteins in social insects. Mol Biol Evol. 2009;26(8):1791-801. Published 2009 Aug. doi:10.1093/molbev/msp086

PMID: 19387012

Citation 4: Fujiwara S, Imai J, Fujiwara M, et al. A potent antibacterial protein in royal jelly. Purification and determination of the primary structure of royalisin. J Biol Chem. 1990;265(19):11333-7. Published 1990 Jul 5. doi:

PMID: 2358464

5.2 Protein Sequence Databases

UniProt: P17722

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: P17722

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AY496432	GenBank \|\| EMBL
2.	U15955	GenBank \|\| EMBL
3.	FJ546136	GenBank \|\| EMBL
4.	AJ308527	GenBank \|\| EMBL

CCDS: Not found

RefSeq: NP_001011616.2

5.5 Protein-Protein Interaction Databases

STRING: 7460.GB41428-PA

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR017982, IPR001542, IPR003614, IPR036574

PANTHER: Not found

PROSITE: PS51378

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: ame:406143

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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