AMPDB_3729 | Lycosin-I

1 General Description

AMPDB ID: AMPDB_3729

Protein Names: Lycosin-I (Anticancer peptide) (Cell penetrating peptide)

Protein Family: Cationic peptide 04 (cupiennin) family; 05 subfamily

Gene Name: Nil

Source Organism: Lycosa singoriensis (Wolf spider) (Aranea singoriensis)

Protein Length: 24 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

RKGWFKAMKSIAKFIAKEKLKEHL

FASTA format

Counts of Amino Acids

'A': 3, 'R': 1, 'N': 0, 'D': 0, 'C': 0, 'Q': 0, 'E': 2, 'G': 1, 'H': 1, 'I': 2, 'L': 2, 'K': 7, 'M': 1, 'F': 2, 'P': 0, 'S': 1, 'T': 0, 'W': 1, 'Y': 0, 'V': 0

Frequencies of Amino Acids

'A': 12.5%, 'R': 4.17%, 'N': 0%, 'D': 0%, 'C': 0%, 'Q': 0%, 'E': 8.33%, 'G': 4.17%, 'H': 4.17%, 'I': 8.33%, 'L': 8.33%, 'K': 29.17%, 'M': 4.17%, 'F': 8.33%, 'P': 0%, 'S': 4.17%, 'T': 0%, 'W': 4.17%, 'Y': 0%, 'V': 0%

Missing Amino Acid(s)

C, D, N, P, Q, T, V, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

G, H, M, R, S, W

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	2888.56 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	77.5	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	-5.292	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.608	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.696	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	11.185	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	6.09	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.292, 0.083, 0.333 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.125	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	5500, 5500	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Acinetobacter baumannii (Gram-negative), Candida species, T.gondii

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Fungicide, Anti-candida, Anti-plasmodium, Anti-gram-negative

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Anti-cancer, Signal peptide, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Liu Z, Deng M, Xiang J, et al. A novel spider peptide toxin suppresses tumor growth through dual signaling pathways. Curr Mol Med. 2012;12(10):1350-60. Published 2012 Dec. doi:10.2174/156652412803833643

PMID: 22882120

Citation 2: Tan H, Ding X, Meng S, et al. Antimicrobial potential of lycosin-I, a cationic and amphiphilic peptide from the venom of the spider Lycosa singorensis. Curr Mol Med. 2013;13(6):900-10. Published 2013 Jul. doi:10.2174/15665240113139990045

PMID: 23638903

Citation 3: Wang L, Wang YJ, Liu YY, et al. In vitro potential of Lycosin-I as an alternative antimicrobial drug for treatment of multidrug-resistant Acinetobacter baumannii infections. Antimicrob Agents Chemother. 2014;58(11):6999-7002. Published 2014 Nov. doi:10.1128/AAC.03279-14

PMID: 25199777

Citation 4: Tan H, Luo W, Wei L, et al. Quantifying the Distribution of the Stoichiometric Composition of Anticancer Peptide Lycosin-I on the Lipid Membrane with Single Molecule Spectroscopy. J Phys Chem B. 2016;120(12):3081-8. Published 2016 Mar 31. doi:10.1021/acs.jpcb.5b12618

PMID: 26937786

Citation 5: Tan H, Huang Y, Xu J, et al. Spider Toxin Peptide Lycosin-I Functionalized Gold Nanoparticles for in vivo Tumor Targeting and Therapy. Theranostics. 2017;7(12):3168-3178. Published 2017. doi:10.7150/thno.19780

PMID: 28839471

Citation 6: Tan L, Bai L, Wang L, et al. Antifungal activity of spider venom-derived peptide lycosin-I against Candida tropicalis. Microbiol Res. 2018;216:120-128. Published 2018 Nov. doi:10.1016/j.micres.2018.08.012

PMID: 30269852

Citation 7: Ma B, Xi Z, Li J, et al. Vasodilator and hypotensive effects of the spider peptide Lycosin-I in vitro and in vivo. Peptides. 2018;99:108-114. Published 2018 Jan. doi:10.1016/j.peptides.2017.12.011

PMID: 29248696

Citation 8: Tang Y, Hou S, Li X, et al. Anti-parasitic effect on Toxoplasma gondii induced by a spider peptide lycosin-I. Exp Parasitol. 2019;198:17-25. Published 2019 Mar. doi:10.1016/j.exppara.2019.01.009

PMID: 30682337

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: P0DV70

5.4 Nucleotide Sequence Databases

No entries found in GenBank or EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: Not found

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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