AMPDB_360 | Galactose N-acetylgalactosamine-binding lectin CEL-III
PEPTIDE SUMMARY
Galactose N-acetylgalactosamine-binding lectin CEL-III
1 General Description
AMPDB ID: AMPDB_360
Protein Names: Galactose N-acetylgalactosamine-binding lectin CEL-III (Gal GalNAc-binding lectin CEL-III) (CEL-III) (Hemolytic lectin CEL-III) (Lectin CEL-III)
Protein Family: Nil
Gene Name: cel3
Protein Length: 442 AA
Protein Existence: Evidence at protein level
2 Protein Sequence & Composition
2.1 Sequence
MVSLVPCGFAQVLCTNPLDIGELRNYKSKQCVDIVGNQGSGNIATHDCDGLSDQQIIMCGDGTIRNEARNYCFTPDGSGNANVMSSPCTLYPEIPSSQRWRLGRKKAFTDNGGIEQVATEIINLASGKCLDVEGSDGTGDIGVYDCQNLDDQYFYIRSRGPELFYGRLRNEKSDLCLDVEGSEGKGNVLMYSCEDNLDQWFRYYENGEIVNAKQGMCLDVEGSDGSGNVGIYRCDDLRDQMWSRPNAYCNGDYCSFLNKESNKCLDVSGDQGTGDVGTWQCDGLPDQRFKWVFDDWEVPTATWNMVGCDQNGKVSQQISNTISFSSTVTAGVAVEVSSTIEKGVIFAKASVSVKVTASLSKAWTNSQSGTTAITYTCDNYDSDEEFTRGCMWQLAIETTEVKSGDLLVWNPQIIKCTRSNTAPGCAPFTKCANEDCTFCTDI
FASTA format
2.2 Composition
Counts of Amino Acids
'A': 21, 'R': 17, 'N': 29, 'D': 38, 'C': 25, 'Q': 21, 'E': 23, 'G': 43, 'H': 1, 'I': 23, 'L': 25, 'K': 19, 'M': 8, 'F': 14, 'P': 13, 'S': 37, 'T': 30, 'W': 10, 'Y': 15, 'V': 30
Frequencies of Amino Acids
'A': 4.75%, 'R': 3.85%, 'N': 6.56%, 'D': 8.6%, 'C': 5.66%, 'Q': 4.75%, 'E': 5.2%, 'G': 9.73%, 'H': 0.23%, 'I': 5.2%, 'L': 5.66%, 'K': 4.3%, 'M': 1.81%, 'F': 3.17%, 'P': 2.94%, 'S': 8.37%, 'T': 6.79%, 'W': 2.26%, 'Y': 3.39%, 'V': 6.79%
Missing Amino Acid(s)
No Amino Acid(s) are missing in this protein
Most Occurring Amino Acid(s)
G
Less Occurring Amino Acid(s)
H
Hydrophobic Amino Acid(s) Count
187
Hydrophilic Amino Acid(s) Count
255
Basic Amino Acid(s) Count
61
Acidic Amino Acid(s) Count
37
Modified Amino Acid(s) Count
0
Modified Amino Acid(s) Frequencies
0
Computed by biopython (version 1.79) & proteinAnalysis (version 1)
3 Physicochemical Properties
Sl. No. Properties Values Reference
1. Molecular Mass 48455.8 Da Computed by ProtParam module (biopython 1.79)
2. Aliphatic Index 66.787 Computed by ProtParam module (biopython 1.79)
3. Instability Index (Half Life) 39.485 Computed by ProtParam module (biopython 1.79)
4. Hydrophobicity (GRAVY) -0.408 Computed by ProtParam module (biopython 1.79)
5. Hydrophobic Moment 0.649 Computed by ProtParam module (biopython 1.79)
6. Isoelectric Point 4.172 Computed by ProtParam module (biopython 1.79)
7. Charge (at pH 7) -26.421 Computed by ProtParam module (biopython 1.79)
8. Secondary Structure Fraction 0.265, 0.276, 0.174 [Helix, Turn, Sheet] Computed by ProtParam module (biopython 1.79)
9. Aromaticity 0.088 Computed by ProtParam module (biopython 1.79)
10. Molar Extinction Coefficient (cysteine|cystine) 77350, 78850 Computed by ProtParam module (biopython 1.79)
4 Activity Details
4.1 Target Organism(s)
A.stephensi, African green, P.berghei, P.falciparum
4.2 Antimicrobial Activity
Antibiotic, Antimicrobial, Anti-malarial, Anti-parasitic
4.3 Enzymatic Activity
Not found
4.4 Inhibitory Effect
Not found
4.5 Other Biological Activity
Cytolysis, Lectin, Cytotoxin, Hemolytic, Non-ribosomal
Activity data manually curated from Literature and UniProt
5 Database Cross-references
5.1 Literature Database
5.1.1 PubMed
Citation 1: Nakano M, Tabata S, Sugihara K, et al. Primary structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata and its cDNA: structural similarity to the B-chain from plant lectin, ricin. Biochim Biophys Acta. 1999;1435(1-2):167-76. Published 1999 Nov 16. doi:10.1016/s0167-4838(99)00212-5
PMID: 10561549
Citation 2: Kouzuma Y, Suzuki Y, Nakano M, et al. Characterization of functional domains of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata. J Biochem. 2003;134(3):395-402. Published 2003 Sep. doi:10.1093/jb/mvg157
PMID: 14561725
Citation 3: Sallay I, Tojo S, Nomiyama K, et al. Calcium ions stabilize a protein structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata. Biosci Biotechnol Biochem. 2001;65(6):1347-52. Published 2001 Jun. doi:10.1271/bbb.65.1347
PMID: 11471734
Citation 4: Hatakeyama T, Kohzaki H, Nagatomo H, et al. Purification and characterization of four Ca(2+)-dependent lectins from the marine invertebrate, Cucumaria echinata. J Biochem. 1994;116(1):209-14. Published 1994 Jul. doi:10.1093/oxfordjournals.jbchem.a124495
PMID: 7798179
Citation 5: Hatakeyama T, Nagatomo H, Yamasaki N, et al. Interaction of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata with the erythrocyte membrane. J Biol Chem. 1995;270(8):3560-4. Published 1995 Feb 24. doi:10.1074/jbc.270.8.3560
PMID: 7876091
Citation 6: Hatakeyama T, Furukawa M, Nagatomo H, et al. Oligomerization of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata induced by the binding of carbohydrate ligands. J Biol Chem. 1996;271(28):16915-20. Published 1996 Jul 12. doi:10.1074/jbc.271.28.16915
PMID: 8663224
Citation 7: Fujisawa T, Kuwahara H, Hiromasa Y, et al. Small-angle X-ray scattering study on CEL-III, a hemolytic lectin from Holothuroidea Cucumaria echinata, and its oligomer induced by the binding of specific carbohydrate. FEBS Lett. 1997;414(1):79-83. Published 1997 Sep 1. doi:10.1016/s0014-5793(97)00976-9
PMID: 9305736
Citation 8: Hatakeyama T, Miyamoto Y, Nagatomo H, et al. Carbohydrate-binding properties of the hemolytic lectin CEL-III from the holothuroidea Cucumaria echinata as analyzed using carbohydrate-coated microplate. J Biochem. 1997;121(1):63-7. Published 1997 Jan. doi:10.1093/oxfordjournals.jbchem.a021571
PMID: 9058193
Citation 9: Oda T, Tsuru M, Hatakeyama T, et al. Temperature- and pH-dependent cytotoxic effect of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata on various cell lines. J Biochem. 1997;121(3):560-7. Published 1997 Mar. doi:10.1093/oxfordjournals.jbchem.a021622
PMID: 9133626
Citation 10: Hatakeyama T, Matsuyama Y, Funada T, et al. Chemical modification of the hemolytic lectin CEL-III by succinic anhydride: involvement of amino groups in the oligomerization process. Biosci Biotechnol Biochem. 1998;62(6):1185-9. Published 1998 Jun. doi:10.1271/bbb.62.1185
PMID: 9692203
Citation 11: Sallay I, Hatakeyama T, Yamasaki N, et al. Studies on the carbohydrate binding sites of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata. Biosci Biotechnol Biochem. 1998;62(9):1757-61. Published 1998 Sep. doi:10.1271/bbb.62.1757
PMID: 9805377
Citation 12: Kouriki-Nagatomo H, Hatakeyama T, Jelokhani-Niaraki M, et al. Molecular mechanism for pore-formation in lipid membranes by the hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata. Biosci Biotechnol Biochem. 1999;63(7):1279-84. Published 1999 Jul. doi:10.1271/bbb.63.1279
PMID: 10478454
Citation 13: Hatakeyama T, Sato T, Taira E, et al. Characterization of the interaction of hemolytic lectin CEL-III from the marine invertebrate, Cucumaria echinata, with artificial lipid membranes: involvement of neutral sphingoglycolipids in the pore-forming process. J Biochem. 1999;125(2):277-84. Published 1999 Feb. doi:10.1093/oxfordjournals.jbchem.a022284
PMID: 9990124
Citation 14: Oda T, Shinmura N, Nishioka Y, et al. Effect of the hemolytic lectin CEL-III from Holothuroidea Cucumaria echinata on the ANS fluorescence responses in sensitive MDCK and resistant CHO cells. J Biochem. 1999;125(4):713-20. Published 1999 Apr. doi:10.1093/oxfordjournals.jbchem.a022341
PMID: 10101284
Citation 15: Kuwahara H, Funada T, Hatakeyama T, et al. Effects of chemical modification of carboxyl groups in the hemolytic lectin CEL-III on its hemolytic and carbohydrate-binding activities. Biosci Biotechnol Biochem. 2000;64(6):1278-81. Published 2000 Jun. doi:10.1271/bbb.64.1278
PMID: 10923802
Citation 16: Kuwahara H, Yamasaki T, Hatakeyama T, et al. Oligomerization process of the hemolytic lectin CEL-III purified from a sea cucumber, Cucumaria echinata. J Biochem. 2002;131(5):751-6. Published 2002 May. doi:10.1093/oxfordjournals.jbchem.a003161
PMID: 11983084
Citation 17: Hatakeyama T, Suenaga T, Eto S, et al. Antibacterial activity of peptides derived from the C-terminal region of a hemolytic lectin, CEL-III, from the marine invertebrate Cucumaria echinata. J Biochem. 2004;135(1):65-70. Published 2004 Jan. doi:10.1093/jb/mvh007
PMID: 14999010
Citation 18: Yoshida S, Shimada Y, Kondoh D, et al. Hemolytic C-type lectin CEL-III from sea cucumber expressed in transgenic mosquitoes impairs malaria parasite development. PLoS Pathog. 2007;3(12):e192. Published 2007 Dec. doi:10.1371/journal.ppat.0030192
PMID: 18159942
Citation 19: Hisamatsu K, Tsuda N, Goda S, et al. Characterization of the {alpha}-helix region in domain 3 of the haemolytic lectin CEL-III: implications for self-oligomerization and haemolytic processes. J Biochem. 2008;143(1):79-86. Published 2008 Jan. doi:10.1093/jb/mvm195
PMID: 17965430
Citation 20: Hisamatsu K, Unno H, Goda S, et al. Effects of Ca2+ on refolding of the recombinant hemolytic lectin CEL-III. Biosci Biotechnol Biochem. 2009;73(5):1203-5. Published 2009 May. doi:10.1271/bbb.80793
PMID: 19420692
Citation 21: Hisamatsu K, Unno H, Goda S, et al. Roles of the valine clusters in domain 3 of the hemolytic lectin CEL-III in its oligomerization and hemolytic abilities. Protein Pept Lett. 2009;16(4):411-4. Published 2009. doi:10.2174/092986609787848054
PMID: 19356139
Citation 22: Shimizu Y, Yamazaki H, Yoshida S, et al. Molecular cloning, functional expression, and characterization of isolectin genes of hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata. Biosci Biotechnol Biochem. 2012;76(2):276-82. Published 2012. doi:10.1271/bbb.110635
PMID: 22313748
Citation 23: Unno H, Hisamatsu K, Nagao T, et al. Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69(Pt 4):416-20. Published 2013 Apr 1. doi:10.1107/S1744309113004065
PMID: 23545649
Citation 24: Hisamatsu K, Nagao T, Unno H, et al. Identification of the amino acid residues involved in the hemolytic activity of the Cucumaria echinata lectin CEL-III. Biochim Biophys Acta. 2013;1830(8):4211-7. Published 2013 Aug. doi:10.1016/j.bbagen.2013.04.010
PMID: 23583369
Citation 25: Goda S, Sadakata H, Unno H, et al. Effects of detergents on the oligomeric structures of hemolytic lectin CEL-III as determined by small-angle X-ray scattering. Biosci Biotechnol Biochem. 2013;77(3):679-81. Published 2013. doi:10.1271/bbb.120981
PMID: 23470749
Citation 26: Nagao T, Masaki R, Unno H, et al. Effects of amino acid mutations in the pore-forming domain of the hemolytic lectin CEL-III. Biosci Biotechnol Biochem. 2016;80(10):1966-9. Published 2016 Oct. doi:10.1080/09168451.2016.1176520
PMID: 27101707
Citation 27: Uchida T, Yamasaki T, Eto S, et al. Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata: implications of domain structure for its membrane pore-formation mechanism. J Biol Chem. 2004;279(35):37133-41. Published 2004 Aug 27. doi:10.1074/jbc.M404065200
PMID: 15194688
Citation 28: Hatakeyama T, Unno H, Kouzuma Y, et al. C-type lectin-like carbohydrate recognition of the hemolytic lectin CEL-III containing ricin-type -trefoil folds. J Biol Chem. 2007;282(52):37826-35. Published 2007 Dec 28. doi:10.1074/jbc.M705604200
PMID: 17977832
Citation 29: Unno H, Goda S, Hatakeyama T, et al. Hemolytic lectin CEL-III heptamerizes via a large structural transition from α-helices to a β-barrel during the transmembrane pore formation process. J Biol Chem. 2014;289(18):12805-12. Published 2014 May 2. doi:10.1074/jbc.M113.541896
PMID: 24652284
5.2 Protein Sequence Databases
UniProt: Q868M7
5.3 3D Structure Databases
Sl. no. PDB ID Method Resolution Access Links 3D View
AlphaFoldDB: Q868M7
5.4 Nucleotide Sequence Databases
Sl. no. Accession(s) Access Link(s)
1. AB109017 GenBank || EMBL
CCDS: Not found
RefSeq: Not found
5.5 Protein-Protein Interaction Databases
STRING: Not found
IntAct: Not found
MINT: Not found
DIP: Not found
BioGRID: Not found
5.6 Ligand Databases
BindingDB: Not found
DrugBank: Not found
ChEMBL: Not found
5.7 Family & Domain Databases
PANTHER: Not found
PROSITE: PS50231
5.8 Genome Annotation Databases
Ensembl: Not found
KEGG: Not found
5.9 Phylogenomic Databases
GeneTree: Not found
5.10 Enzyme & Pathway Databases
BRENDA: Not found
BioCyc: Not found
5.11 Protein-RNA Interaction Databases
RNAct: Not found




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