AMPDB_3570 | Colicin-Ia

PEPTIDE SUMMARY

Colicin-Ia

1 General Description

AMPDB ID: AMPDB_3570

Protein Names: Colicin-Ia

Protein Family: Channel forming colicin family

Gene Name: cia

Source Organism: Escherichia coli

Protein Length: 626 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPPAWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRLSAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHTESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNYLLSHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLSGINQKIAEEKRKQDELKATKDAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKINAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRPLFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFWGI

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 77, 'R': 47, 'N': 43, 'D': 38, 'C': 0, 'Q': 22, 'E': 50, 'G': 36, 'H': 6, 'I': 38, 'L': 56, 'K': 50, 'M': 6, 'F': 15, 'P': 14, 'S': 42, 'T': 39, 'W': 7, 'Y': 14, 'V': 26

Frequencies of Amino Acids

'A': 12.3%, 'R': 7.51%, 'N': 6.87%, 'D': 6.07%, 'C': 0%, 'Q': 3.51%, 'E': 7.99%, 'G': 5.75%, 'H': 0.96%, 'I': 6.07%, 'L': 8.95%, 'K': 7.99%, 'M': 0.96%, 'F': 2.4%, 'P': 2.24%, 'S': 6.71%, 'T': 6.23%, 'W': 1.12%, 'Y': 2.24%, 'V': 4.15%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

H, M

Hydrophobic Amino Acid(s) Count

275

Hydrophilic Amino Acid(s) Count

351

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

103

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	69429 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	82.907	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	33.995	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.636	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.754	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.722	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	9.622	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.249, 0.216, 0.302 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.058	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	59360, 59360	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Cell membrane, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Mankovich JA, Hsu CH, Konisky J, et al. DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and Ib. J Bacteriol. 1986;168(1):228-36. Published 1986 Oct. doi:10.1128/jb.168.1.228-236.1986

PMID: 3531169

Citation 2: Riley MA, Tan Y, Wang J, et al. Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli. Proc Natl Acad Sci U S A. 1994;91(23):11276-80. Published 1994 Nov 8. doi:10.1073/pnas.91.23.11276

PMID: 7972047

Citation 3: Wiener M, Freymann D, Ghosh P, et al. Crystal structure of colicin Ia. Nature. 1997;385(6615):461-4. Published 1997 Jan 30. doi:10.1038/385461a0

PMID: 9009197

5.2 Protein Sequence Databases

UniProt: P06716

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P06716

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M13819	GenBank \|\| EMBL
2.	U15622	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_001582575.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: P06716

MINT: P06716

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: DB04147

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR000293, IPR038283, IPR014740, IPR014739

PANTHER: Not found

PROSITE: PS00276

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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