AMPDB_3567 | Colicin-A

PEPTIDE SUMMARY

Colicin-A

1 General Description

AMPDB ID: AMPDB_3567

Protein Names: Colicin-A

Protein Family: Channel forming colicin family

Gene Name: caa

Source Organism: Citrobacter freundii

Protein Length: 592 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MPGFNYGGKGDGTGWSSERGSGPEPGGGSHGNSGGHDRGDSSNVGNESVTVMKPGDSYNTPWGKVIINAAGQPTMNGTVMTADNSSMVPYGRGFTRVLNSLVNNPVSPAGQNGGKSPVQTAVENYLMVQSGNLPPGYWLSNGKVMTEVREERTSGGGGKNGNERTWTVKVPREVPQLTASYNEGMRIRQEAADRARAEANARALAEEEARAIASGKSKAEFDAGKRVEAAQAAINTAQLNVNNLSGAVSAANQVITQKQAEMTPLKNELAAANQRVQETLKFINDPIRSRIHFNMRSGLIRAQHNVDTKQNEINAAVANRDALNSQLSQANNILQNARNEKSAADAALSAATAQRLQAEAALRAAAEAAEKARQRQAEEAERQRQAMEVAEKAKDERELLEKTSELIAGMGDKIGEHLGDKYKAIAKDIADNIKNFQGKTIRSFDDAMASLNKITANPAMKINKADRDALVNAWKHVDAQDMANKLGNLSKAFKVADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASSVALGIFSATLGAYALSLGVPAIAVGIAGILLAAVVGALIDDKFADALNNEIIRPAH

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 88, 'R': 33, 'N': 48, 'D': 26, 'C': 0, 'Q': 26, 'E': 42, 'G': 54, 'H': 7, 'I': 29, 'L': 38, 'K': 36, 'M': 17, 'F': 10, 'P': 20, 'S': 39, 'T': 24, 'W': 7, 'Y': 9, 'V': 39

Frequencies of Amino Acids

'A': 14.86%, 'R': 5.57%, 'N': 8.11%, 'D': 4.39%, 'C': 0%, 'Q': 4.39%, 'E': 7.09%, 'G': 9.12%, 'H': 1.18%, 'I': 4.9%, 'L': 6.42%, 'K': 6.08%, 'M': 2.87%, 'F': 1.69%, 'P': 3.38%, 'S': 6.59%, 'T': 4.05%, 'W': 1.18%, 'Y': 1.52%, 'V': 6.59%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

H, W

Hydrophobic Amino Acid(s) Count

302

Hydrophilic Amino Acid(s) Count

290

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	62992.6 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	78.108	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	26.533	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.457	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.837	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.627	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	1.702	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.223, 0.272, 0.313 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.044	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	51910, 51910	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Cell membrane, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Morlon J, LloubÃ¨s R, Varenne S, et al. Complete nucleotide sequence of the structural gene for colicin A, a gene translated at non-uniform rate. J Mol Biol. 1983;170(2):271-85. Published 1983 Oct 25. doi:10.1016/s0022-2836(83)80148-x

PMID: 6313941

Citation 2: Morlon J, Chartier M, Bidaud M, et al. The complete nucleotide sequence of the colicinogenic plasmid ColA. High extent of homology with ColE1. Mol Gen Genet. 1988;211(2):231-43. Published 1988 Feb. doi:10.1007/BF00330599

PMID: 2832701

Citation 3: Morlon J, Lloubes R, Chartier M, et al. Nucleotide sequence of promoter, operator and amino-terminal region of caa, the structural gene of colicin A. EMBO J. 1983;2(5):787-9. Published 1983. doi:10.1002/j.1460-2075.1983.tb01501.x

PMID: 6641715

5.2 Protein Sequence Databases

UniProt: P04480

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P04480

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	X01008	GenBank \|\| EMBL
2.	M37402	GenBank \|\| EMBL
3.	M26369	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_008323639.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: P04480

MINT: P04480

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR000293, IPR038283

PANTHER: Not found

PROSITE: PS00276

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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