AMPDB_3563 | Colicin-E1

1 General Description

AMPDB ID: AMPDB_3563

Protein Names: Colicin-E1

Protein Family: Channel forming colicin family

Gene Name: cea

Source Organism: Escherichia coli

Protein Length: 522 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTINEVLGI

FASTA format

Counts of Amino Acids

'A': 80, 'R': 27, 'N': 26, 'D': 27, 'C': 1, 'Q': 24, 'E': 46, 'G': 29, 'H': 7, 'I': 28, 'L': 44, 'K': 60, 'M': 5, 'F': 11, 'P': 7, 'S': 32, 'T': 26, 'W': 4, 'Y': 13, 'V': 25

Frequencies of Amino Acids

'A': 15.33%, 'R': 5.17%, 'N': 4.98%, 'D': 5.17%, 'C': 0.19%, 'Q': 4.6%, 'E': 8.81%, 'G': 5.56%, 'H': 1.34%, 'I': 5.36%, 'L': 8.43%, 'K': 11.49%, 'M': 0.96%, 'F': 2.11%, 'P': 1.34%, 'S': 6.13%, 'T': 4.98%, 'W': 0.77%, 'Y': 2.49%, 'V': 4.79%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

233

Hydrophilic Amino Acid(s) Count

289

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	57279.9 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	83.008	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	29.078	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.595	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.78	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.913	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	14.637	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.239, 0.18, 0.335 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.054	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	41370, 41370	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Cell membrane, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Yamada M, Ebina Y, Miyata T, et al. Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein. Proc Natl Acad Sci U S A. 1982;79(9):2827-31. Published 1982 May. doi:10.1073/pnas.79.9.2827

PMID: 6953432

Citation 2: Riley MA, Tan Y, Wang J, et al. Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli. Proc Natl Acad Sci U S A. 1994;91(23):11276-80. Published 1994 Nov 8. doi:10.1073/pnas.91.23.11276

PMID: 7972047

Citation 3: Ebina Y, Kishi F, Miki T, et al. The nucleotide sequence surrounding the promoter region of colicin E1 gene. Gene. 1981;15(2-3):119-26. Published 1981 Nov. doi:10.1016/0378-1119(81)90121-9

PMID: 6271636

Citation 4: Oka A, Nomura N, Morita M, et al. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 1979;172(2):151-9. Published 1979 May 4. doi:10.1007/BF00268276

PMID: 384144

Citation 5: Wormald MR, Merrill AR, Cramer WA, et al. Solution NMR studies of colicin E1 C-terminal thermolytic peptide. Structural comparison with colicin A and the effects of pH changes. Eur J Biochem. 1990;191(1):155-61. Published 1990 Jul 20. doi:10.1111/j.1432-1033.1990.tb19105.x

PMID: 2199197

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P02978

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	J01563	GenBank \|\| EMBL
2.	U15633	GenBank \|\| EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR000293, IPR038283

PANTHER: Not found

PROSITE: PS00276

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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