AMPDB_3546 | Cloacin

PEPTIDE SUMMARY

Cloacin

1 General Description

AMPDB ID: AMPDB_3546

Protein Names: Cloacin (EC 3.1.-.-) (Ribonuclease)

Protein Family: Cloacin colicin family

Gene Name: ccl

Source Organism: Escherichia coli

Protein Length: 561 AA

Protein Existence: Inferred from homology

2 Protein Sequence & Composition

2.1 Sequence

MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSGPGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPALATPGAEGPALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSKIMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKPTKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDAEDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQVRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 57, 'R': 25, 'N': 24, 'D': 35, 'C': 0, 'Q': 22, 'E': 34, 'G': 74, 'H': 10, 'I': 16, 'L': 29, 'K': 58, 'M': 8, 'F': 11, 'P': 42, 'S': 37, 'T': 27, 'W': 6, 'Y': 13, 'V': 33

Frequencies of Amino Acids

'A': 10.16%, 'R': 4.46%, 'N': 4.28%, 'D': 6.24%, 'C': 0%, 'Q': 3.92%, 'E': 6.06%, 'G': 13.19%, 'H': 1.78%, 'I': 2.85%, 'L': 5.17%, 'K': 10.34%, 'M': 1.43%, 'F': 1.96%, 'P': 7.49%, 'S': 6.6%, 'T': 4.81%, 'W': 1.07%, 'Y': 2.32%, 'V': 5.88%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

276

Hydrophilic Amino Acid(s) Count

285

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	59278.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	58.503	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	35.66	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.84	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.644	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.968	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	14.955	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.193, 0.316, 0.228 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.053	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	52370, 52370	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Endonuclease, Hydrolase, Nuclease

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Nijkamp HJ, de Lang R, Stuitje AR, et al. The complete nucleotide sequence of the bacteriocinogenic plasmid CloDF13. Plasmid. 1986;16(2):135-60. Published 1986 Sep. doi:10.1016/0147-619x(86)90072-7

PMID: 3749334

Citation 2: van den Elzen PJ, Walters HH, Veltkamp E, et al. Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13. Nucleic Acids Res. 1983;11(8):2465-77. Published 1983 Apr 25. doi:10.1093/nar/11.8.2465

PMID: 6344017

5.2 Protein Sequence Databases

UniProt: P00645

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: P00645

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	X04466	GenBank \|\| EMBL

CCDS: Not found

RefSeq: NP_052372.1, WP_010891190.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR024575, IPR036725, IPR009105, IPR024566, IPR016128, IPR036302

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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