AMPDB_343 | L-amino-acid oxidase

1 General Description

AMPDB ID: AMPDB_343

Protein Names: L-amino-acid oxidase (LAAO) (LAO) (EC 1.4.3.2) (Escapin)

Protein Family: Flavin monoamine oxidase family

Gene Name: Nil

Source Organism: Aplysia californica (California sea hare)

Protein Length: 535 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MSSAFLLLACALVISVHADGVCRNRRQCNKEVCGSSYDVAIVGAGPGGANSAYMLRESGLDIAVFEYSDRVGGRLFTYQLPNTPDVNLEIGGMRFIEGAMHRLWKVISELGLTPKVFKEGFGKEGRQRFYLRGQSLTKKQVKSGDVPYDLSPEEKANQGRLVEYYLEKLTGLQLNGGPLKREVALKLTVPDGRFLYDLTFDEALDLVASPEGKEFARDTHVFTSEVTLDASAISIFDDHLGEDYYGSEIYTLEEGMSSVPQGLLQTFLDAAKSNEFFPNNHLKALRRRTNGQYVLYFEPTTSKDGQTTINYLEPLKVVCAQRVILAMPVYALRQLDWSQLRNDRATQAYRAVRPMPASKVFMTFDQPWWLQNERKSWVTKSDALFSQMYDWQKSEASGDYILIASYADGLKAQYLRELKNQGEDIPGSDPGYNQVTVPLKDAILEHLTEAYGVERDSIPEPVTAASQFWTDYPFGCGWITWRAGYHFDDVISTMRRPSLKDEVYVVGSDYSWGLISSWIEGALETSENVINDYFL

FASTA format

Counts of Amino Acids

'A': 38, 'R': 30, 'N': 18, 'D': 35, 'C': 6, 'Q': 23, 'E': 36, 'G': 42, 'H': 7, 'I': 21, 'L': 55, 'K': 25, 'M': 10, 'F': 23, 'P': 24, 'S': 39, 'T': 27, 'W': 11, 'Y': 28, 'V': 37

Frequencies of Amino Acids

'A': 7.1%, 'R': 5.61%, 'N': 3.36%, 'D': 6.54%, 'C': 1.12%, 'Q': 4.3%, 'E': 6.73%, 'G': 7.85%, 'H': 1.31%, 'I': 3.93%, 'L': 10.28%, 'K': 4.67%, 'M': 1.87%, 'F': 4.3%, 'P': 4.49%, 'S': 7.29%, 'T': 5.05%, 'W': 2.06%, 'Y': 5.23%, 'V': 6.92%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

261

Hydrophilic Amino Acid(s) Count

274

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	60300.1 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	82.561	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	43.829	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.323	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.715	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	4.843	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-15.689	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.327, 0.23, 0.26 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.116	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	102220, 102595	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

B.subtilis (Gram-positive), C.sphaerospermum, E.coli (Gram-negative), P.aeruginosa (Gram-negative), S.aureus (Gram-positive), S.pyogenes (Gram-positive), S.typhimurium, V.harveyi (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Fungicide, Anti-yeast, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Yang H, Johnson PM, Ko KC, et al. Cloning, characterization and expression of escapin, a broadly antimicrobial FAD-containing L-amino acid oxidase from ink of the sea hare Aplysia californica. J Exp Biol. 2005;208(Pt 18):3609-22. Published 2005 Sep. doi:10.1242/jeb.01795

PMID: 16155232

Citation 2: Johnson PM, Kicklighter CE, Schmidt M, et al. Packaging of chemicals in the defensive secretory glands of the sea hare Aplysia californica. J Exp Biol. 2006;209(Pt 1):78-88. Published 2006 Jan. doi:10.1242/jeb.01972

PMID: 16354780

Citation 3: Ko KC, Wang B, Tai PC, et al. Identification of potent bactericidal compounds produced by escapin, an L-amino acid oxidase in the ink of the sea hare Aplysia californica. Antimicrob Agents Chemother. 2008;52(12):4455-62. Published 2008 Dec. doi:10.1128/AAC.01103-08

PMID: 18852282

Citation 4: Kamio M, Ko KC, Zheng S, et al. The chemistry of escapin: identification and quantification of the components in the complex mixture generated by an L-amino acid oxidase in the defensive secretion of the sea snail Aplysia californica. Chemistry. 2009;15(7):1597-603. Published 2009. doi:10.1002/chem.200801696

PMID: 19130530

Citation 5: Nusnbaum M, Derby CD, Derby CD. Effects of sea hare ink secretion and its escapin-generated components on a variety of predatory fishes. Biol Bull. 2010;218(3):282-92. Published 2010 Jun. doi:10.1086/BBLv218n3p282

PMID: 20570851

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: Q6IWZ0

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AY615888	GenBank \|\| EMBL

RefSeq: NP_001191570.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR002937, IPR036188

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

Download