AMPDB_32713 | Colicin E8

PEPTIDE SUMMARY

Colicin E8

1 General Description

AMPDB ID: AMPDB_32713

Protein Names: Colicin E8

Protein Family: Colicin pyosin nuclease family

Gene Name: ce8a

Source Organism: Escherichia coli

Protein Length: 573 AA

Protein Existence: Inferred from homology

2 Protein Sequence & Composition

2.1 Sequence

MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAADADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHIDIHRGQ

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 57, 'R': 35, 'N': 38, 'D': 45, 'C': 0, 'Q': 23, 'E': 26, 'G': 64, 'H': 11, 'I': 23, 'L': 27, 'K': 43, 'M': 8, 'F': 15, 'P': 33, 'S': 48, 'T': 24, 'W': 7, 'Y': 4, 'V': 42

Frequencies of Amino Acids

'A': 9.95%, 'R': 6.11%, 'N': 6.63%, 'D': 7.85%, 'C': 0%, 'Q': 4.01%, 'E': 4.54%, 'G': 11.17%, 'H': 1.92%, 'I': 4.01%, 'L': 4.71%, 'K': 7.5%, 'M': 1.4%, 'F': 2.62%, 'P': 5.76%, 'S': 8.38%, 'T': 4.19%, 'W': 1.22%, 'Y': 0.7%, 'V': 7.33%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

276

Hydrophilic Amino Acid(s) Count

297

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	60872.4 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	65.236	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	40.88	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.742	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.729	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.867	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	8.048	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.206, 0.319, 0.206 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.045	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	44460, 44460	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Hydrolase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Le Gac M, Doebeli M, Doebeli M. Environmental viscosity does not affect the evolution of cooperation during experimental evolution of colicigenic bacteria. Evolution. 2010;64(2):522-33. Published 2010 Feb 1. doi:10.1111/j.1558-5646.2009.00814.x

PMID: 19674096

5.2 Protein Sequence Databases

UniProt: C6GJY4

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: C6GJY4

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	FJ985252	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_012766032.1, YP_002993419.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR024575, IPR037146, IPR044925, IPR003615, IPR016128, IPR036302

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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