AMPDB_32541 | Colicin E9

PEPTIDE SUMMARY

Colicin E9

1 General Description

AMPDB ID: AMPDB_32541

Protein Names: Colicin E9

Protein Family: Colicin pyosin nuclease family

Gene Name: ceaI

Source Organism: Escherichia coli

Protein Length: 582 AA

Protein Existence: Inferred from homology

2 Protein Sequence & Composition

2.1 Sequence

MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNDSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEEVSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIRVTTPKRHIDIHRGK

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 59, 'R': 30, 'N': 37, 'D': 45, 'C': 0, 'Q': 22, 'E': 28, 'G': 63, 'H': 11, 'I': 23, 'L': 25, 'K': 47, 'M': 8, 'F': 14, 'P': 34, 'S': 53, 'T': 24, 'W': 7, 'Y': 6, 'V': 46

Frequencies of Amino Acids

'A': 10.14%, 'R': 5.15%, 'N': 6.36%, 'D': 7.73%, 'C': 0%, 'Q': 3.78%, 'E': 4.81%, 'G': 10.82%, 'H': 1.89%, 'I': 3.95%, 'L': 4.3%, 'K': 8.08%, 'M': 1.37%, 'F': 2.41%, 'P': 5.84%, 'S': 9.11%, 'T': 4.12%, 'W': 1.2%, 'Y': 1.03%, 'V': 7.9%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

279

Hydrophilic Amino Acid(s) Count

303

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	61587.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	65.223	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	37.837	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.715	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.729	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.45	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	5.049	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.208, 0.321, 0.206 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.046	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	47440, 47440	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative

4.3 Enzymatic Activity

Endonuclease, Hydrolase, Nuclease

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Cooper PC, James R, James R. Two new E colicins, E8 and E9, produced by a strain of Escherichia coli. J Gen Microbiol. 1984;130(1):209-15. Published 1984 Jan. doi:10.1099/00221287-130-1-209

PMID: 6368743

Citation 2: Chak KF, James R, James R. Characterization of the ColE9-J plasmid and analysis of its genetic organization. J Gen Microbiol. 1986;132(1):61-70. Published 1986 Jan. doi:10.1099/00221287-132-1-61

PMID: 3011960

5.2 Protein Sequence Databases

UniProt: B9VM99

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: B9VM99

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	FJ573246	GenBank \|\| EMBL

CCDS: Not found

RefSeq: WP_012644886.1, YP_002533537.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR024575, IPR037146, IPR024566, IPR044925, IPR003615, IPR016128, IPR036302

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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