AMPDB_325 | Frenatin 4.1

PEPTIDE SUMMARY

Frenatin 4.1

1 General Description

AMPDB ID: AMPDB_325

Protein Names: Frenatin 4.1 [Cleaved into: Frenatin 4.2]

Protein Family: Frog skin active peptide (FSAP) family; Frenatin subfamily

Gene Name: Nil

Source Organism: Nyctimystes infrafrenatus (White-lipped tree frog) (Litoria infrafrenata)

Protein Length: 70 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MAFLKKSLFLVLFLGLVNLSICEEEKREEENKEEEDENEALSEVKRGFLEKLKTGAKDFASAFVNSIKGT

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 5, 'R': 2, 'N': 4, 'D': 2, 'C': 1, 'Q': 0, 'E': 13, 'G': 4, 'H': 0, 'I': 2, 'L': 10, 'K': 9, 'M': 1, 'F': 6, 'P': 0, 'S': 5, 'T': 2, 'W': 0, 'Y': 0, 'V': 4

Frequencies of Amino Acids

'A': 7.14%, 'R': 2.86%, 'N': 5.71%, 'D': 2.86%, 'C': 1.43%, 'Q': 0%, 'E': 18.57%, 'G': 5.71%, 'H': 0%, 'I': 2.86%, 'L': 14.29%, 'K': 12.86%, 'M': 1.43%, 'F': 8.57%, 'P': 0%, 'S': 7.14%, 'T': 2.86%, 'W': 0%, 'Y': 0%, 'V': 5.71%

Missing Amino Acid(s)

H, P, Q, W, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

C, M

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	7942.09 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	90.571	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	45.359	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.337	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.651	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	4.531	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-4.043	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.314, 0.186, 0.414 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.086	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 0	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

C.albicans, E.coli (Gram-negative)

4.2 Antimicrobial Activity

Amphibian defense peptide, Antibiotic, Antimicrobial, Anti-candida, Anti-gram-negative

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Zhou M, Chen T, Walker B, et al. Novel frenatins from the skin of the Australasian giant white-lipped tree frog, Litoria infrafrenata: cloning of precursor cDNAs and identification in defensive skin secretion. Peptides. 2005;26(12):2445-51. Published 2005 Dec. doi:10.1016/j.peptides.2005.05.019

PMID: 15996792

Citation 2: Wu D, Gao Y, Wang L, et al. A Combined Molecular Cloning and Mass Spectrometric Method to Identify, Characterize, and Design Frenatin Peptides from the Skin Secretion of Litoria infrafrenata. Molecules. 2016;21(11). Published 2016 Oct 26. doi:10.3390/molecules21111429

PMID: 27792198

5.2 Protein Sequence Databases

UniProt: Q571V3

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: Q571V3

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AJ937526	GenBank \|\| EMBL

CCDS: Not found

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR004275, IPR016322

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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