AMPDB_294 | Guanylate-binding protein 1

1 General Description

AMPDB ID: AMPDB_294

Protein Names: Guanylate-binding protein 1 (EC 3.6.5.-) (GTP-binding protein 1) (GBP-1) (mGBP-1) (mGBP1) (Guanine nucleotide-binding protein 1) (Interferon-gamma-inducible protein MAG-1) (Interferon-induced guanylate-binding protein 1)

Protein Family: TRAFAC class dynamin-like GTPase superfamily; GB1 RHD3 GTPase family; GB1 subfamily

Gene Name: Gbp1 Gbp-1 Mag-1 Mpa1

Source Organism: Mus musculus (Mouse)

Protein Length: 589 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MASEIHMSEPMCLIENTEAQLVINQEALRILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKRTGFSLGSTVQSHTKGIWMWCVPHPKKAGQTLVLLDTEGLEDVEKGDNQNDCWIFALAVLLSSTFIYNSIGTINQQAMDQLHYVTELTDLIKSKSSPDQSDVDNSANFVGFFPIFVWTLRDFSLDLEFDGESITPDEYLETSLALRKGTDENTKKFNMPRLCIRKFFPKRKCFIFDRPGDRKQLSKLEWIQEDQLNKEFVEQVAEFTSYIFSYSGVKTLSGGITVNGPRLKSLVQTYVSAICSGELPCMENAVLTLAQIENSAAVQKAITYYEEQMNQKIHMPTETLQELLDLHRTCEREAIEVFMKNSFKDVDQKFQEELGAQLEAKRDAFVKKNMDMSSAHCSDLLEGLFAHLEEEVKQGTFYKPGGYYLFLQRKQELEKKYIQTPGKGLQAEVMLRKYFESKEDLADTLLKMDQSLTEKEKQIEMERIKAEAAEAANRALAEMQKKHEMLMEQKEQSYQEHMKQLTEKMEQERKELMAEQQRIISLKLQEQERLLKQGFQNESLQLRQEIEKIKNMPPPRSCTIL

FASTA format

Counts of Amino Acids

'A': 35, 'R': 23, 'N': 21, 'D': 26, 'C': 10, 'Q': 44, 'E': 62, 'G': 27, 'H': 10, 'I': 32, 'L': 64, 'K': 50, 'M': 23, 'F': 26, 'P': 18, 'S': 37, 'T': 32, 'W': 5, 'Y': 16, 'V': 28

Frequencies of Amino Acids

'A': 5.94%, 'R': 3.9%, 'N': 3.57%, 'D': 4.41%, 'C': 1.7%, 'Q': 7.47%, 'E': 10.53%, 'G': 4.58%, 'H': 1.7%, 'I': 5.43%, 'L': 10.87%, 'K': 8.49%, 'M': 3.9%, 'F': 4.41%, 'P': 3.06%, 'S': 6.28%, 'T': 5.43%, 'W': 0.85%, 'Y': 2.72%, 'V': 4.75%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

258

Hydrophilic Amino Acid(s) Count

331

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	67711.7 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	83.294	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	46.616	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.465	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.625	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	5.226	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-14.619	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.29, 0.175, 0.312 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.08	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	51340, 51965	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Listeria monocytogenes (Gram-positive), Mycobacterium bovis (Gram-positive), Toxoplasma gondii

4.2 Antimicrobial Activity

Antimicrobial, Anti-listeria, Anti-protozoal, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Cell membrane, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Wynn TA, Nicolet CM, Paulnock DM, et al. Identification and characterization of a new gene family induced during macrophage activation. J Immunol. 1991;147(12):4384-92. Published 1991 Dec 15. doi:

PMID: 1753106

Citation 2: Cheng YS, Patterson CE, Staeheli P, et al. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP. Mol Cell Biol. 1991;11(9):4717-25. Published 1991 Sep. doi:10.1128/mcb.11.9.4717-4725.1991

PMID: 1715024

Citation 3: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 4: Stickney JT, Buss JE, Buss JE. Murine guanylate-binding protein: incomplete geranylgeranyl isoprenoid modification of an interferon-gamma-inducible guanosine triphosphate-binding protein. Mol Biol Cell. 2000;11(7):2191-200. Published 2000 Jul. doi:10.1091/mbc.11.7.2191

PMID: 10888661

Citation 5: Degrandi D, Konermann C, Beuter-Gunia C, et al. Extensive characterization of IFN-induced GTPases mGBP1 to mGBP10 involved in host defense. J Immunol. 2007;179(11):7729-40. Published 2007 Dec 1. doi:10.4049/jimmunol.179.11.7729

PMID: 18025219

Citation 6: Huttlin EL, Jedrychowski MP, Elias JE, et al. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010;143(7):1174-89. Published 2010 Dec 23. doi:10.1016/j.cell.2010.12.001

PMID: 21183079

Citation 7: Kim BH, Shenoy AR, Kumar P, et al. A family of IFN-Î³-inducible 65-kD GTPases protects against bacterial infection. Science. 2011;332(6030):717-21. Published 2011 May 6. doi:10.1126/science.1201711

PMID: 21551061

5.2 Protein Sequence Databases

5.3 3D Structure Databases

AlphaFoldDB: Q01514

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M63961	GenBank \|\| EMBL
2.	M55544	GenBank \|\| EMBL
3.	BC108990	GenBank \|\| EMBL

CCDS: CCDS38658.1

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: Not found

PROSITE: PS51715

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

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