AMPDB_288 | Spiderine-1a

PEPTIDE SUMMARY

Spiderine-1a

1 General Description

AMPDB ID: AMPDB_288

Protein Names: Spiderine-1a (OtTx1a)

Protein Family: Spiderine family; Cationic spiderine subfamily

Gene Name: Nil

Source Organism: Oxyopes takobius (Lynx spider) (Oxyopes foliiformis)

Protein Length: 166 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MKFALVLLGVCAFYLVNATGDLETELEASELQELQEALDLIGETPLESLEAEELEEARKFKWGKLFSTAKKLYKKGKKLSKNKNFKKALKFGKQLAKNLQAGEEHEPGTPVGNNKCWAIGTTCSDDCDCCPEHHCHCPAGKWLPGLFRCTCQVTESDKVNKCPPAE

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 14, 'R': 2, 'N': 7, 'D': 6, 'C': 11, 'Q': 5, 'E': 19, 'G': 12, 'H': 4, 'I': 2, 'L': 21, 'K': 21, 'M': 1, 'F': 7, 'P': 8, 'S': 6, 'T': 9, 'W': 3, 'Y': 2, 'V': 6

Frequencies of Amino Acids

'A': 8.43%, 'R': 1.2%, 'N': 4.22%, 'D': 3.61%, 'C': 6.63%, 'Q': 3.01%, 'E': 11.45%, 'G': 7.23%, 'H': 2.41%, 'I': 1.2%, 'L': 12.65%, 'K': 12.65%, 'M': 0.6%, 'F': 4.22%, 'P': 4.82%, 'S': 3.61%, 'T': 5.42%, 'W': 1.81%, 'Y': 1.2%, 'V': 3.61%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

K, L

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	18421.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	72.952	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	40.595	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.476	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.724	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	6.49	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-2.292	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.247, 0.199, 0.331 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.072	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	19480, 20105	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

A.globiformis (Gram-positive), B.subtilis (Gram-positive), E.coli (Gram-negative), E.faecalis (Gram-positive), P.aeruginosa (Gram-negative), S.aureus (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Cytolysis, Toxin, Cytolytic, Cytotoxin, Insecticidal, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Vassilevski AA, Sachkova MY, Ignatova AA, et al. Spider toxins comprising disulfide-rich and linear amphipathic domains: a new class of molecules identified in the lynx spider Oxyopes takobius. FEBS J. 2013;280(23):6247-61. Published 2013 Dec. doi:10.1111/febs.12547

PMID: 24118933

Citation 2: Nadezhdin KD, Romanovskaia DD, Sachkova MY, et al. Modular toxin from the lynx spider Oxyopes takobius: Structure of spiderine domains in solution and membrane-mimicking environment. Protein Sci. 2017;26(3):611-616. Published 2017 Mar. doi:10.1002/pro.3101

PMID: 27997708

5.2 Protein Sequence Databases

UniProt: P86716

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P86716

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	JX134895	GenBank \|\| EMBL

CCDS: Not found

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR044061

PANTHER: Not found

PROSITE: PS51861

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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