AMPDB_28 | Lactoperoxidase

PEPTIDE SUMMARY

Lactoperoxidase

1 General Description

AMPDB ID: AMPDB_28

Protein Names: Lactoperoxidase (LPO) (WBLP) (EC 1.11.1.7)

Protein Family: Peroxidase family; XPO subfamily

Gene Name: LPO

Source Organism: Bubalus bubalis (Domestic water buffalo)

Protein Length: 712 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGQTRTAIRNGQVWEESFKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLTRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKFSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 49, 'R': 45, 'N': 39, 'D': 38, 'C': 16, 'Q': 34, 'E': 37, 'G': 42, 'H': 15, 'I': 31, 'L': 78, 'K': 40, 'M': 12, 'F': 37, 'P': 44, 'S': 43, 'T': 45, 'W': 16, 'Y': 16, 'V': 35

Frequencies of Amino Acids

'A': 6.88%, 'R': 6.32%, 'N': 5.48%, 'D': 5.34%, 'C': 2.25%, 'Q': 4.78%, 'E': 5.2%, 'G': 5.9%, 'H': 2.11%, 'I': 4.35%, 'L': 10.96%, 'K': 5.62%, 'M': 1.69%, 'F': 5.2%, 'P': 6.18%, 'S': 6.04%, 'T': 6.32%, 'W': 2.25%, 'Y': 2.25%, 'V': 4.92%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

344

Hydrophilic Amino Acid(s) Count

368

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

100

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	80698.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	80.843	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	36.88	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.387	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.864	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.596	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	10.427	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.299, 0.236, 0.247 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.097	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	111840, 112840	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

E.coli (Gram-negative), K.pneumoniae (Gram-negative), P.aeruginosa (Gram-negative), S.dysenteriae (Gram-negative), S.epidermidis (Gram-positive), S.sonnei (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Oxidoreductase, Peroxidase

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Ozdemir H, Hacibeyoglu HI, Uslu H, et al. Purification of lactoperoxidase from creek-water buffalo milk and investigation of kinetic and antibacterial properties. Prep Biochem Biotechnol. 2002;32(2):143-55. Published 2002 May. doi:10.1081/PB-120004127

PMID: 12071645

Citation 2: B S GK, Mohan Reddy P, Kottekad S, et al. Comparative Site-Specific N-Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC-MS/MS Reveals a Distinct Glycan Pattern. J Agric Food Chem. 2018;66(43):11492-11499. Published 2018 Oct 31. doi:10.1021/acs.jafc.8b03243

PMID: 30296068

Citation 3: Sheikh IA, Singh AK, Singh N, et al. Structural evidence of substrate specificity in mammalian peroxidases: structure of the thiocyanate complex with lactoperoxidase and its interactions at 2.4 A resolution. J Biol Chem. 2009;284(22):14849-56. Published 2009 May 29. doi:10.1074/jbc.M807644200

PMID: 19339248

5.2 Protein Sequence Databases

UniProt: A5JUY8

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: A5JUY8

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	EF580919	GenBank \|\| EMBL
2.	HQ285238	GenBank \|\| EMBL

CCDS: Not found

RefSeq: NP_001277812.1

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR019791, IPR010255, IPR037120, IPR029587

PANTHER: PTHR11475:SF67

PROSITE: PS50292

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: bbub:102405711

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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