AMPDB_260 | Uperin-3.5

PEPTIDE SUMMARY

Uperin-3.5

1 General Description

AMPDB ID: AMPDB_260

Protein Names: Uperin-3.5

Protein Family: Nil

Gene Name: Nil

Source Organism: Uperoleia mjobergii (Mjoberg`s toadlet) (Pseudophryne mjobergii)

Protein Length: 17 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

GVGDLIRKAVSVIKNIV

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 1, 'R': 1, 'N': 1, 'D': 1, 'C': 0, 'Q': 0, 'E': 0, 'G': 2, 'H': 0, 'I': 3, 'L': 1, 'K': 2, 'M': 0, 'F': 0, 'P': 0, 'S': 1, 'T': 0, 'W': 0, 'Y': 0, 'V': 4

Frequencies of Amino Acids

'A': 5.88%, 'R': 5.88%, 'N': 5.88%, 'D': 5.88%, 'C': 0%, 'Q': 0%, 'E': 0%, 'G': 11.76%, 'H': 0%, 'I': 17.65%, 'L': 5.88%, 'K': 11.76%, 'M': 0%, 'F': 0%, 'P': 0%, 'S': 5.88%, 'T': 0%, 'W': 0%, 'Y': 0%, 'V': 23.53%

Missing Amino Acid(s)

C, E, F, H, M, P, Q, T, W, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

A, D, L, N, R, S

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	1781.17 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	165.882	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	20.277	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	0.882	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.784	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	10.789	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	1.998	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.471, 0.235, 0.118 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 0	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

B.cereus (Gram-positive), L.innocua (Gram-positive), L.lactis (Gram-positive), M.luteus (Gram-negative), S.aureus (Gram-positive), S.epidermidis (Gram-positive), S.hominis (Gram-negative), S.uberis (Gram-positive)

4.2 Antimicrobial Activity

Amphibian defense peptide, Antibiotic, Antimicrobial, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Calabrese AN, Liu Y, Wang T, et al. The Amyloid Fibril-Forming Properties of the Amphibian Antimicrobial Peptide Uperinâ3.5. Chembiochem. 2016;17(3):239-46. Published 2016 Feb 2. doi:10.1002/cbic.201500518

PMID: 26676975

Citation 2: John T, Dealey TJA, Gray NP, et al. The Kinetics of Amyloid Fibrillar Aggregation of Uperin 3.5 Is Directed by the Peptide's Secondary Structure. Biochemistry. 2019;58(35):3656-3668. Published 2019 Sep 3. doi:10.1021/acs.biochem.9b00536

PMID: 31385514

Citation 3: Salinas N, Tayeb-Fligelman E, Sammito MD, et al. The amphibian antimicrobial peptide uperin 3.5 is a cross-Î±/cross-Î² chameleon functional amyloid. Proc Natl Acad Sci U S A. 2021;118(3). Published 2021 Jan 19. doi:10.1073/pnas.2014442118

PMID: 33431675

5.2 Protein Sequence Databases

UniProt: P82042

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P82042

5.4 Nucleotide Sequence Databases

No entries found in GenBank or EMBL

CCDS: Not found

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

MINT: Not found

DIP: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR012527

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

KEGG: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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