AMPDB_24 | Macrophage-expressed gene 1 protein

1 General Description

AMPDB ID: AMPDB_24

Protein Names: Macrophage-expressed gene 1 protein (Macrophage gene 1 protein) (Mpg-1) (Perforin-2) (P-2) (Protein MPS1)

Protein Family: MPEG1 family

Gene Name: Mpeg1

Source Organism: Mus musculus (Mouse)

Protein Length: 713 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MNSFMALVLIWMIIACAEADKPLGETGTTGFQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKTTPTSELSLGFTKALMDICDQLEKNQTKMATYLAELLILNYGTHVITSVDAGAALVQEDHVRSSFLLDNQNSQNTVTASAGIAFLNIVNFKVETDYISQTSLTKDYLSNRTNSRVQSFGGVPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMDDDSCDAKVTNFTFGGVYQECTELSGDVLCQNLEQKNLLTGDFSCPPGYSPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAEFRAYWCVAAGQVPDNSGLLFGGVFTDKTINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGFKFSVPFGGFFSCIMGNPLVNSDTAKDVRAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTNSETARSWIKDPQTNQWKLGEPLELRRAMTVIHGDSNGMSGGEAAGITLGVTIALGIVITLAIYGTRKYKKKEYQEIEEQESLVGSLATDATVLNGEEDPSPA

FASTA format

Counts of Amino Acids

'A': 42, 'R': 21, 'N': 40, 'D': 34, 'C': 20, 'Q': 30, 'E': 39, 'G': 53, 'H': 10, 'I': 34, 'L': 69, 'K': 42, 'M': 16, 'F': 32, 'P': 35, 'S': 49, 'T': 63, 'W': 7, 'Y': 23, 'V': 54

Frequencies of Amino Acids

'A': 5.89%, 'R': 2.95%, 'N': 5.61%, 'D': 4.77%, 'C': 2.81%, 'Q': 4.21%, 'E': 5.47%, 'G': 7.43%, 'H': 1.4%, 'I': 4.77%, 'L': 9.68%, 'K': 5.89%, 'M': 2.24%, 'F': 4.49%, 'P': 4.91%, 'S': 6.87%, 'T': 8.84%, 'W': 0.98%, 'Y': 3.23%, 'V': 7.57%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

342

Hydrophilic Amino Acid(s) Count

371

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	78390.5 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	84.194	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	33.296	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.14	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.73	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	5.715	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-10.28	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.307, 0.248, 0.233 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.087	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	72770, 74020	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

L.monocytogenes (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 2: Spilsbury K, O'Mara MA, Wu WM, et al. Isolation of a novel macrophage-specific gene by differential cDNA analysis. Blood. 1995;85(6):1620-9. Published 1995 Mar 15. doi:

PMID: 7888681

Citation 3: Huttlin EL, Jedrychowski MP, Elias JE, et al. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010;143(7):1174-89. Published 2010 Dec 23. doi:10.1016/j.cell.2010.12.001

PMID: 21183079

Citation 4: Fields KA, McCormack R, de Armas LR, et al. Perforin-2 restricts growth of Chlamydia trachomatis in macrophages. Infect Immun. 2013;81(8):3045-54. Published 2013 Aug. doi:10.1128/IAI.00497-13

PMID: 23753625

Citation 5: McCormack R, de Armas LR, Shiratsuchi M, et al. Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1. J Innate Immun. 2013;5(2):185-94. Published 2013. doi:10.1159/000345249

PMID: 23257510

Citation 6: McCormack RM, Lyapichev K, Olsson ML, et al. Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2. Elife. 2015;4. Published 2015 Sep 29. doi:10.7554/eLife.06505

PMID: 26418746

Citation 7: McCormack RM, de Armas LR, Shiratsuchi M, et al. Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. Elife. 2015;4. Published 2015 Sep 24. doi:10.7554/eLife.06508

PMID: 26402460

Citation 8: McCormack R, Bahnan W, Shrestha N, et al. Perforin-2 Protects Host Cells and Mice by Restricting the Vacuole to Cytosol Transitioning of a Bacterial Pathogen. Infect Immun. 2016;84(4):1083-1091. Published 2016 Apr. doi:10.1128/IAI.01434-15

PMID: 26831467

Citation 9: Bai F, McCormack RM, Hower S, et al. Perforin-2 Breaches the Envelope of Phagocytosed Bacteria Allowing Antimicrobial Effectors Access to Intracellular Targets. J Immunol. 2018;201(9):2710-2720. Published 2018 Nov 1. doi:10.4049/jimmunol.1800365

PMID: 30249808

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: A1L314

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	BC129843	GenBank \|\| EMBL
2.	BC129844	GenBank \|\| EMBL
3.	L20315	GenBank \|\| EMBL

RefSeq: NP_034951.1

5.5 Protein-Protein Interaction Databases

IntAct: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR020864, IPR039707

PANTHER: PTHR31463

PROSITE: PS51412

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

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