AMPDB_212 | Gallinacin-2

1 General Description

AMPDB ID: AMPDB_212

Protein Names: Gallinacin-2 (Gal-2) (Beta-defensin 2)

Protein Family: Beta-defensin family

Gene Name: GAL2

Source Organism: Gallus gallus (Chicken)

Protein Length: 64 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MRILYLLFSLLFLALQVSPGLSSPRRDMLFCKGGSCHFGGCPSHLIKVGSCFGFRSCCKWPWNA

FASTA format

Counts of Amino Acids

'A': 2, 'R': 4, 'N': 1, 'D': 1, 'C': 6, 'Q': 1, 'E': 0, 'G': 7, 'H': 2, 'I': 2, 'L': 10, 'K': 3, 'M': 2, 'F': 6, 'P': 4, 'S': 8, 'T': 0, 'W': 2, 'Y': 1, 'V': 2

Frequencies of Amino Acids

'A': 3.13%, 'R': 6.25%, 'N': 1.56%, 'D': 1.56%, 'C': 9.38%, 'Q': 1.56%, 'E': 0%, 'G': 10.94%, 'H': 3.13%, 'I': 3.13%, 'L': 15.63%, 'K': 4.69%, 'M': 3.13%, 'F': 9.38%, 'P': 6.25%, 'S': 12.5%, 'T': 0%, 'W': 3.13%, 'Y': 1.56%, 'V': 3.13%

Missing Amino Acid(s)

E, T

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

D, N, Q, Y

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	7141.56 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	85.313	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	71.77	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	0.458	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.518	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.206	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	5.807	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.359, 0.313, 0.219 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.141	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	12490, 12865	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

E.coli (Gram-negative), L.monocytogenes (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Defensin, Anti-candida, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Brockus CW, Jackwood MW, Harmon BG, et al. Characterization of beta-defensin prepropeptide mRNA from chicken and turkey bone marrow. Anim Genet. 1998;29(4):283-9. Published 1998 Aug. doi:10.1046/j.1365-2052.1998.00338.x

PMID: 9745666

Citation 2: Xiao Y, Hughes AL, Ando J, et al. A genome-wide screen identifies a single beta-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensins. BMC Genomics. 2004;5(1):56. Published 2004 Aug 13. doi:10.1186/1471-2164-5-56

PMID: 15310403

Citation 3: Harwig SS, Swiderek KM, Kokryakov VN, et al. Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett. 1994;342(3):281-5. Published 1994 Apr 11. doi:10.1016/0014-5793(94)80517-2

PMID: 8150085

Citation 4: Kannan L, Rath NC, Liyanage R, et al. Direct screening identifies mature beta-defensin 2 in avian heterophils. Poult Sci. 2009;88(2):372-9. Published 2009 Feb. doi:10.3382/ps.2008-00366

PMID: 19151352

Citation 5: Lynn DJ, Higgs R, Gaines S, et al. Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken. Immunogenetics. 2004;56(3):170-7. Published 2004 Jun. doi:10.1007/s00251-004-0675-0

PMID: 15148642

Citation 6: Yoshimura Y, Ohashi H, Subedi K, et al. Effects of age, egg-laying activity, and Salmonella-inoculation on the expressions of gallinacin mRNA in the vagina of the hen oviduct. J Reprod Dev. 2006;52(2):211-8. Published 2006 Apr. doi:10.1262/jrd.17070

PMID: 16394622

Citation 7: Subedi K, Isobe N, Nishibori M, et al. Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus). Reproduction. 2007;133(1):127-33. Published 2007 Jan. doi:10.1530/REP-06-0083

PMID: 17244739

Citation 8: Derache C, Meudal H, Aucagne V, et al. Initial insights into structure-activity relationships of avian defensins. J Biol Chem. 2012;287(10):7746-55. Published 2012 Mar 2. doi:10.1074/jbc.M111.312108

PMID: 22205704

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P46158

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	AF033336	GenBank \|\| EMBL
2.	AY621317	GenBank \|\| EMBL
3.	AY672652	GenBank \|\| EMBL
4.	DQ677633	GenBank \|\| EMBL
5.	DQ858299	GenBank \|\| EMBL
6.	DQ858312	GenBank \|\| EMBL
7.	DQ858325	GenBank \|\| EMBL
8.	DQ858339	GenBank \|\| EMBL

5.5 Protein-Protein Interaction Databases

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: IPR001855

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: ENSGT00530000068084

KEGG: gga:395840

5.9 Phylogenomic Databases

GeneTree: ENSGT00530000068084

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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