AMPDB_200 | Guanylate-binding protein 2

1 General Description

AMPDB ID: AMPDB_200

Protein Names: Guanylate-binding protein 2 (EC 3.6.5.-) (GTP-binding protein 2) (GBP-2) (HuGBP-2) (Guanine nucleotide-binding protein 2) (Interferon-induced guanylate-binding protein 2)

Protein Family: TRAFAC class dynamin-like GTPase superfamily; GB1 RHD3 GTPase family; GB1 subfamily

Gene Name: GBP2

Source Organism: Homo sapiens (Human)

Protein Length: 591 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MAPEINLPGPMSLIDNTKGQLVVNPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKKNGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLKEGFENESKRLQKDIWDIQMRSKSLEPICNIL

FASTA format

Counts of Amino Acids

'A': 39, 'R': 21, 'N': 25, 'D': 34, 'C': 9, 'Q': 35, 'E': 57, 'G': 27, 'H': 9, 'I': 29, 'L': 67, 'K': 58, 'M': 17, 'F': 23, 'P': 24, 'S': 40, 'T': 24, 'W': 6, 'Y': 14, 'V': 33

Frequencies of Amino Acids

'A': 6.6%, 'R': 3.55%, 'N': 4.23%, 'D': 5.75%, 'C': 1.52%, 'Q': 5.92%, 'E': 9.64%, 'G': 4.57%, 'H': 1.52%, 'I': 4.91%, 'L': 11.34%, 'K': 9.81%, 'M': 2.88%, 'F': 3.89%, 'P': 4.06%, 'S': 6.77%, 'T': 4.06%, 'W': 1.02%, 'Y': 2.37%, 'V': 5.58%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

265

Hydrophilic Amino Acid(s) Count

326

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	67209 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	86.142	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	40.524	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.485	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.702	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	5.411	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-11.654	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.291, 0.196, 0.305 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.073	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	53860, 54360	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

4.2 Antimicrobial Activity

Antimicrobial, Anti-protozoal

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Cheng YS, Patterson CE, Staeheli P, et al. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP. Mol Cell Biol. 1991;11(9):4717-25. Published 1991 Sep. doi:10.1128/mcb.11.9.4717-4725.1991

PMID: 1715024

Citation 2: Bechtel S, Rosenfelder H, Duda A, et al. The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007;8:399. Published 2007 Oct 31. doi:10.1186/1471-2164-8-399

PMID: 17974005

Citation 3: Ota T, Suzuki Y, Nishikawa T, et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004;36(1):40-5. Published 2004 Jan. doi:10.1038/ng1285

PMID: 14702039

Citation 4: Gregory SG, Barlow KF, McLay KE, et al. The DNA sequence and biological annotation of human chromosome 1. Nature. 2006;441(7091):315-21. Published 2006 May 18. doi:10.1038/nature04727

PMID: 16710414

Citation 5: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 6: Neun R, Richter MF, Staeheli P, et al. GTPase properties of the interferon-induced human guanylate-binding protein 2. FEBS Lett. 1996;390(1):69-72. Published 1996 Jul 15. doi:10.1016/0014-5793(96)00628-x

PMID: 8706832

Citation 7: Tripal P, Bauer M, Naschberger E, et al. Unique features of different members of the human guanylate-binding protein family. J Interferon Cytokine Res. 2007;27(1):44-52. Published 2007 Jan. doi:10.1089/jir.2007.0086

PMID: 17266443

Citation 8: Britzen-Laurent N, Bauer M, Berton V, et al. Intracellular trafficking of guanylate-binding proteins is regulated by heterodimerization in a hierarchical manner. PLoS One. 2010;5(12):e14246. Published 2010 Dec 7. doi:10.1371/journal.pone.0014246

PMID: 21151871

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P32456

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M55543	GenBank \|\| EMBL
2.	AL832451	GenBank \|\| EMBL
3.	AK314325	GenBank \|\| EMBL
4.	CR457062	GenBank \|\| EMBL
5.	AC104459	GenBank \|\| EMBL
6.	CH471097	GenBank \|\| EMBL
7.	BC073163	GenBank \|\| EMBL

RefSeq: NP_004111.2

5.5 Protein-Protein Interaction Databases

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: Not found

PROSITE: PS51715

5.8 Genome Annotation Databases

Ensembl: ENSGT00940000162297

5.9 Phylogenomic Databases

GeneTree: ENSGT00940000162297

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

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