AMPDB_19 | Lactoperoxidase

1 General Description

AMPDB ID: AMPDB_19

Protein Names: Lactoperoxidase (LPO) (EC 1.11.1.7)

Protein Family: Peroxidase family; XPO subfamily

Gene Name: LPO

Source Organism: Capra hircus (Goat)

Protein Length: 712 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MLVCLHLQVFLASVALFEVAASDTIAQAASTTTISDAVSKVKTQVNKAFLDSRTRLKTALSSEAPTTRQLSEYFKHAKGRTRTAIRNGQVWEESLKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLAVPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSSEHSKVQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLARDQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYPPFNNVKPSPCEFINTTAHVPCFQAGDSRASEQILLATVHTLLLREHNRLARELKRLNPHWDGEMLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLLAKKSKLMNQNKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQAVLKNKILAKKLLDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFSRLICDNTHVTKVPLHAFQANNYPHDFVDCSAVDKLDLSPWASREN

FASTA format

Counts of Amino Acids

'A': 52, 'R': 47, 'N': 38, 'D': 39, 'C': 16, 'Q': 33, 'E': 36, 'G': 42, 'H': 17, 'I': 29, 'L': 78, 'K': 37, 'M': 12, 'F': 34, 'P': 44, 'S': 45, 'T': 40, 'W': 15, 'Y': 16, 'V': 42

Frequencies of Amino Acids

'A': 7.3%, 'R': 6.6%, 'N': 5.34%, 'D': 5.48%, 'C': 2.25%, 'Q': 4.63%, 'E': 5.06%, 'G': 5.9%, 'H': 2.39%, 'I': 4.07%, 'L': 10.96%, 'K': 5.2%, 'M': 1.69%, 'F': 4.78%, 'P': 6.18%, 'S': 6.32%, 'T': 5.62%, 'W': 2.11%, 'Y': 2.25%, 'V': 5.9%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

348

Hydrophilic Amino Acid(s) Count

364

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

101

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	80365.9 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	83.02	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	37.447	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.354	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.896	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.513	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	9.608	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.301, 0.237, 0.25 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.091	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	106340, 107340	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

A.niger, C.cassicola, E.coli (Gram-negative), P.aeruginosa (Gram-negative), P.meadii, Salmonella species (Gram-negative), Staphylococcus species (Gram-positive), Trichoderma species

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-candida, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Oxidoreductase, Peroxidase

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Singh AK, Singh N, Sharma S, et al. Crystal structure of lactoperoxidase at 2.4 A resolution. J Mol Biol. 2008;376(4):1060-75. Published 2008 Feb 29. doi:10.1016/j.jmb.2007.12.012

PMID: 18191143

Citation 2: Benoy MJ, Essy AK, Sreekumar B, et al. Thiocyanate mediated antifungal and antibacterial property of goat milk lactoperoxidase. Life Sci. 2000;66(25):2433-9. Published 2000. doi:10.1016/s0024-3205(00)80003-x

PMID: 10894086

Citation 3: B S GK, Mohan Reddy P, Kottekad S, et al. Comparative Site-Specific N-Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC-MS/MS Reveals a Distinct Glycan Pattern. J Agric Food Chem. 2018;66(43):11492-11499. Published 2018 Oct 31. doi:10.1021/acs.jafc.8b03243

PMID: 30296068

Citation 4: Singh RP, Singh A, Kushwaha GS, et al. Mode of binding of the antithyroid drug propylthiouracil to mammalian haem peroxidases. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 3):304-10. Published 2015 Mar. doi:10.1107/S2053230X15001806

PMID: 25760705

Citation 5: Singh RP, Singh A, Kushwaha GS, et al. Retraction: Mode of binding of the antithyroid drug propylthiouracil to mammalian haem peroxidases. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 6):804. Published 2015 Jun. doi:10.1107/S2053230X15006962

PMID: 26057817

Citation 6: Singh RP, Singh A, Sirohi HV, et al. Dual binding mode of antithyroid drug methimazole to mammalian heme peroxidases - structural determination of the lactoperoxidase-methimazole complex at 1.97 Ã resolution. FEBS Open Bio. 2016;6(7):640-50. Published 2016 Jul. doi:10.1002/2211-5463.12051

PMID: 27398304

5.2 Protein Sequence Databases

UniProt: A0A452E9Y6

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: A0A452E9Y6

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	EF363153	GenBank \|\| EMBL
2.	LWLT01000022	GenBank \|\| EMBL

5.5 Protein-Protein Interaction Databases

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: PTHR11475:SF67

PROSITE: PS50292

5.8 Genome Annotation Databases

Ensembl: ENSGT00940000160488

KEGG: chx:100860761

5.9 Phylogenomic Databases

GeneTree: ENSGT00940000160488

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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