AMPDB_167 | Bactericidal permeability-increasing protein

1 General Description

AMPDB ID: AMPDB_167

Protein Names: Bactericidal permeability-increasing protein (BPI) (CAP 57)

Protein Family: BPI LBP Plunc superfamily; BPI LBP family

Gene Name: BPI

Source Organism: Homo sapiens (Human)

Protein Length: 487 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MRENMARGPCNAPRWASLMVLVAIGTAVTAAVNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSELQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYK

FASTA format

Counts of Amino Acids

'A': 32, 'R': 15, 'N': 24, 'D': 16, 'C': 4, 'Q': 21, 'E': 20, 'G': 28, 'H': 14, 'I': 27, 'L': 49, 'K': 35, 'M': 18, 'F': 25, 'P': 34, 'S': 45, 'T': 23, 'W': 3, 'Y': 14, 'V': 40

Frequencies of Amino Acids

'A': 6.57%, 'R': 3.08%, 'N': 4.93%, 'D': 3.29%, 'C': 0.82%, 'Q': 4.31%, 'E': 4.11%, 'G': 5.75%, 'H': 2.87%, 'I': 5.54%, 'L': 10.06%, 'K': 7.19%, 'M': 3.7%, 'F': 5.13%, 'P': 6.98%, 'S': 9.24%, 'T': 4.72%, 'W': 0.62%, 'Y': 2.87%, 'V': 8.21%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

Hydrophobic Amino Acid(s) Count

256

Hydrophilic Amino Acid(s) Count

231

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	53899.7 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	91.253	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	43.202	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.048	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.698	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	9.89	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	15.043	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.324, 0.269, 0.244 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.086	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	37360, 37610	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

P.aeruginosa (Gram-negative)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Anti-gram-negative

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Cytotoxin, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Gray PW, Flaggs G, Leong SR, et al. Cloning of the cDNA of a human neutrophil bactericidal protein. Structural and functional correlations. J Biol Chem. 1989;264(16):9505-9. Published 1989 Jun 5. doi:

PMID: 2722846

Citation 2: Wilde CG, Seilhamer JJ, McGrogan M, et al. Bactericidal/permeability-increasing protein and lipopolysaccharide (LPS)-binding protein. LPS binding properties and effects on LPS-mediated cell activation. J Biol Chem. 1994;269(26):17411-6. Published 1994 Jul 1. doi:

PMID: 7517398

Citation 3: Ota T, Suzuki Y, Nishikawa T, et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004;36(1):40-5. Published 2004 Jan. doi:10.1038/ng1285

PMID: 14702039

Citation 4: Deloukas P, Matthews LH, Ashurst J, et al. The DNA sequence and comparative analysis of human chromosome 20. Nature. 2001;414(6866):865-71. Published 2001 Dec 20-27. doi:10.1038/414865a

PMID: 11780052

Citation 5: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 6: Wasiluk KR, Skubitz KM, Gray BH, et al. Comparison of granule proteins from human polymorphonuclear leukocytes which are bactericidal toward Pseudomonas aeruginosa. Infect Immun. 1991;59(11):4193-200. Published 1991 Nov. doi:10.1128/iai.59.11.4193-4200.1991

PMID: 1937776

Citation 7: Gabay JE, Scott RW, Campanelli D, et al. Antibiotic proteins of human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1989;86(14):5610-4. Published 1989 Jul. doi:10.1073/pnas.86.14.5610

PMID: 2501794

Citation 8: Ooi CE, Weiss J, Elsbach P, et al. A 25-kDa NH2-terminal fragment carries all the antibacterial activities of the human neutrophil 60-kDa bactericidal/permeability-increasing protein. J Biol Chem. 1987;262(31):14891-4. Published 1987 Nov 5. doi:

PMID: 3667613

Citation 9: Horwitz AH, Leigh SD, Abrahamson S, et al. Expression and characterization of cysteine-modified variants of an amino-terminal fragment of bactericidal/permeability-increasing protein. Protein Expr Purif. 1996;8(1):28-40. Published 1996 Aug. doi:10.1006/prep.1996.0071

PMID: 8812832

Citation 10: Beamer LJ, Carroll SF, Eisenberg D, et al. Crystal structure of human BPI and two bound phospholipids at 2.4 angstrom resolution. Science. 1997;276(5320):1861-4. Published 1997 Jun 20. doi:10.1126/science.276.5320.1861

PMID: 9188532

Citation 11: Kleiger G, Beamer LJ, Grothe R, et al. The 1.7 A crystal structure of BPI: a study of how two dissimilar amino acid sequences can adopt the same fold. J Mol Biol. 2000;299(4):1019-34. Published 2000 Jun 16. doi:10.1006/jmbi.2000.3805

PMID: 10843855

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P17213

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	J04739	GenBank \|\| EMBL
2.	DQ414688	GenBank \|\| EMBL
3.	AK315328	GenBank \|\| EMBL
4.	AL359555	GenBank \|\| EMBL
5.	AL391095	GenBank \|\| EMBL
6.	AL499625	GenBank \|\| EMBL
7.	AL583962	GenBank \|\| EMBL
8.	BC040955	GenBank \|\| EMBL
9.	AF322588	GenBank \|\| EMBL

RefSeq: NP_001716.2

5.5 Protein-Protein Interaction Databases

5.6 Ligand Databases

BindingDB: Not found

DrugBank: DB04178

ChEMBL: Not found

5.7 Family & Domain Databases

PROSITE: PS00400

5.8 Genome Annotation Databases

Ensembl: ENSGT01070000253841

5.9 Phylogenomic Databases

GeneTree: ENSGT01070000253841

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

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