AMPDB_155 | Defensin alpha 4

1 General Description

AMPDB ID: AMPDB_155

Protein Names: Defensin alpha 4 (Corticostatin HP-4) (HNP-4) (HP-4) (Neutrophil defensin 4)

Protein Family: Alpha-defensin family

Gene Name: DEFA4 DEF4

Source Organism: Homo sapiens (Human)

Protein Length: 97 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MRIIALLAAILLVALQVRAGPLQARGDEAPGQEQRGPEDQDISISFAWDKSSALQVSGSTRGMVCSCRLVFCRRTELRVGNCLIGGVSFTYCCTRVD

FASTA format

Counts of Amino Acids

'A': 9, 'R': 10, 'N': 1, 'D': 5, 'C': 6, 'Q': 6, 'E': 4, 'G': 9, 'H': 0, 'I': 6, 'L': 10, 'K': 1, 'M': 2, 'F': 3, 'P': 3, 'S': 8, 'T': 4, 'W': 1, 'Y': 1, 'V': 8

Frequencies of Amino Acids

'A': 9.28%, 'R': 10.31%, 'N': 1.03%, 'D': 5.15%, 'C': 6.19%, 'Q': 6.19%, 'E': 4.12%, 'G': 9.28%, 'H': 0%, 'I': 6.19%, 'L': 10.31%, 'K': 1.03%, 'M': 2.06%, 'F': 3.09%, 'P': 3.09%, 'S': 8.25%, 'T': 4.12%, 'W': 1.03%, 'Y': 1.03%, 'V': 8.25%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

L, R

Less Occurring Amino Acid(s)

K, N, W, Y

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	10504.2 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	97.526	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	46.096	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	0.178	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.589	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.112	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	1.634	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.299, 0.216, 0.258 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.052	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	6990, 7365	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

B.anthracis (Gram-positive), B.cereus (Gram-positive), C.albicans, E.coli (Gram-negative), S.aureus (Gram-positive), S.faecalis (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Defensin, Fungicide, Anti-HIV, Anti-candida, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Palfree RG, Sadro LC, Solomon S, et al. The gene encoding the human corticostatin HP-4 precursor contains a recent 86-base duplication and is located on chromosome 8. Mol Endocrinol. 1993;7(2):199-205. Published 1993 Feb. doi:10.1210/mend.7.2.8469233

PMID: 8469233

Citation 2: Nusbaum C, Mikkelsen TS, Zody MC, et al. DNA sequence and analysis of human chromosome 8. Nature. 2006;439(7074):331-5. Published 2006 Jan 19. doi:10.1038/nature04406

PMID: 16421571

Citation 3: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 4: Singh A, Bateman A, Zhu QZ, et al. Structure of a novel human granulocyte peptide with anti-ACTH activity. Biochem Biophys Res Commun. 1988;155(1):524-9. Published 1988 Aug 30. doi:10.1016/s0006-291x(88)81118-5

PMID: 2843187

Citation 5: Wilde CG, Griffith JE, Marra MN, et al. Purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. J Biol Chem. 1989;264(19):11200-3. Published 1989 Jul 5. doi:

PMID: 2500436

Citation 6: Gabay JE, Scott RW, Campanelli D, et al. Antibiotic proteins of human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1989;86(14):5610-4. Published 1989 Jul. doi:10.1073/pnas.86.14.5610

PMID: 2501794

Citation 7: Wu Z, Ericksen B, Tucker K, et al. Synthesis and characterization of human alpha-defensins 4-6. J Pept Res. 2004;64(3):118-25. Published 2004 Sep. doi:10.1111/j.1399-3011.2004.00179.x

PMID: 15317502

Citation 8: Ericksen B, Wu Z, Lu W, et al. Antibacterial activity and specificity of the six human {alpha}-defensins. Antimicrob Agents Chemother. 2005;49(1):269-75. Published 2005 Jan. doi:10.1128/AAC.49.1.269-275.2005

PMID: 15616305

Citation 9: Wu Z, Cocchi F, Gentles D, et al. Human neutrophil alpha-defensin 4 inhibits HIV-1 infection in vitro. FEBS Lett. 2005;579(1):162-6. Published 2005 Jan 3. doi:10.1016/j.febslet.2004.11.062

PMID: 15620707

Citation 10: Szyk A, Wu Z, Tucker K, et al. Crystal structures of human alpha-defensins HNP4, HD5, and HD6. Protein Sci. 2006;15(12):2749-60. Published 2006 Dec. doi:10.1110/ps.062336606

PMID: 17088326

Citation 11: Hu H, Di B, Tolbert WD, et al. Systematic mutational analysis of human neutrophil Î±-defensin HNP4. Biochim Biophys Acta Biomembr. 2019;1861(4):835-844. Published 2019 Apr 1. doi:10.1016/j.bbamem.2019.01.007

PMID: 30658057

Citation 12: SjÃ¶blom T, Jones S, Wood LD, et al. The consensus coding sequences of human breast and colorectal cancers. Science. 2006;314(5797):268-74. Published 2006 Oct 13. doi:10.1126/science.1133427

PMID: 16959974

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P12838

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	U18745	GenBank \|\| EMBL
2.	AF200455	GenBank \|\| EMBL
3.	CH471153	GenBank \|\| EMBL
4.	BC093959	GenBank \|\| EMBL
5.	BC106747	GenBank \|\| EMBL
6.	BC112091	GenBank \|\| EMBL
7.	X65977	GenBank \|\| EMBL

CCDS: CCDS5961.1

RefSeq: NP_001916.1

5.5 Protein-Protein Interaction Databases

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PANTHER: PTHR11876

PROSITE: PS00269

5.8 Genome Annotation Databases

Ensembl: ENSGT00940000153268

5.9 Phylogenomic Databases

GeneTree: ENSGT00940000153268

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

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