AMPDB_147 | Ovoinhibitor
PEPTIDE SUMMARY
Ovoinhibitor
1 General Description
AMPDB ID: AMPDB_147
Protein Names: Ovoinhibitor (OI) (OvoI) (Serine protease inhibitor Kazal-type 5) (allergen Gal d OIH)
Protein Family: Nil
Gene Name: SPINK5 OIH
Source Organism: Gallus gallus (Chicken)
Protein Length: 517 AA
Protein Existence: Evidence at protein level
2 Protein Sequence & Composition
2.1 Sequence
MTDWVLHHKVGPLDMTTRYIFPLLPLPFLPHSESKRAVCAPRCSAMRTARQFVQVALALCCFADIAFGIEVNCSLYASGIGKDGTSWVACPRNLKPVCGTDGSTYSNECGICLYNREHGANVEKEYDGECRPKHVTIDCSPYLQVVRDGNTMVACPRILKPVCGSDSFTYDNECGICAYNAEHHTNISKLHDGECKLEIGSVDCSKYPSTVSKDGRTLVACPRILSPVCGTDGFTYDNECGICAHNAEQRTHVSKKHDGKCRQEIPEIDCDQYPTRKTTGGKLLVRCPRILLPVCGTDGFTYDNECGICAHNAQHGTEVKKSHDGRCKERSTPLDCTQYLSNTQNGEAITACPFILQEVCGTDGVTYSNDCSLCAHNIELGTSVAKKHDGRCREEVPELDCSKYKTSTLKDGRQVVACTMIYDPVCATNGVTYASECTLCAHNLEQRTNLGKRKNGRCEEDITKEHCREFQKVSPICTMEYVPHCGSDGVTYSNRCFFCNAYVQSNRTLNLVSMAAC
FASTA format
2.2 Composition
Counts of Amino Acids
'A': 30, 'R': 28, 'N': 25, 'D': 30, 'C': 46, 'Q': 14, 'E': 31, 'G': 38, 'H': 19, 'I': 22, 'L': 33, 'K': 28, 'M': 7, 'F': 12, 'P': 25, 'S': 32, 'T': 40, 'W': 2, 'Y': 20, 'V': 35
Frequencies of Amino Acids
'A': 5.8%, 'R': 5.42%, 'N': 4.84%, 'D': 5.8%, 'C': 8.9%, 'Q': 2.71%, 'E': 6%, 'G': 7.35%, 'H': 3.68%, 'I': 4.26%, 'L': 6.38%, 'K': 5.42%, 'M': 1.35%, 'F': 2.32%, 'P': 4.84%, 'S': 6.19%, 'T': 7.74%, 'W': 0.39%, 'Y': 3.87%, 'V': 6.77%
Missing Amino Acid(s)
No Amino Acid(s) are missing in this protein
Most Occurring Amino Acid(s)
C
Less Occurring Amino Acid(s)
W
Hydrophobic Amino Acid(s) Count
204
Hydrophilic Amino Acid(s) Count
313
Basic Amino Acid(s) Count
61
Acidic Amino Acid(s) Count
75
Modified Amino Acid(s) Count
0
Modified Amino Acid(s) Frequencies
0
Computed by biopython (version 1.79) & proteinAnalysis (version 1)
3 Physicochemical Properties
Sl. No. Properties Values Reference
1. Molecular Mass 57004.7 Da Computed by ProtParam module (biopython 1.79)
2. Aliphatic Index 66.925 Computed by ProtParam module (biopython 1.79)
3. Instability Index (Half Life) 45.626 Computed by ProtParam module (biopython 1.79)
4. Hydrophobicity (GRAVY) -0.378 Computed by ProtParam module (biopython 1.79)
5. Hydrophobic Moment 0.737 Computed by ProtParam module (biopython 1.79)
6. Isoelectric Point 6.843 Computed by ProtParam module (biopython 1.79)
7. Charge (at pH 7) -6.083 Computed by ProtParam module (biopython 1.79)
8. Secondary Structure Fraction 0.24, 0.232, 0.195 [Helix, Turn, Sheet] Computed by ProtParam module (biopython 1.79)
9. Aromaticity 0.066 Computed by ProtParam module (biopython 1.79)
10. Molar Extinction Coefficient (cysteine|cystine) 40800, 43675 Computed by ProtParam module (biopython 1.79)
4 Activity Details
4.1 Target Organism(s)
Aspergillus oryzae, Drosophila
4.2 Antimicrobial Activity
Antimicrobial
4.3 Enzymatic Activity
Not found
4.4 Inhibitory Effect
Protease inhibitor, Serine protease inhibitor
4.5 Other Biological Activity
Enzyme inhibitor, Non-hemolytic, Non-ribosomal
Activity data manually curated from Literature and UniProt
5 Database Cross-references
5.1 Literature Database
5.1.1 PubMed
Citation 1: International Chicken Genome Sequencing Consortium. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature. 2004;432(7018):695-716. Published 2004 Dec 9. doi:10.1038/nature03154
PMID: 15592404
Citation 2: Scott MJ, Huckaby CS, Kato I, et al. Ovoinhibitor introns specify functional domains as in the related and linked ovomucoid gene. J Biol Chem. 1987;262(12):5899-907. Published 1987 Apr 25. doi:
PMID: 3571241
Citation 3: Kim J, Choi YH, Choi YH. Differential abundance of egg white proteins in laying hens treated with corticosterone. J Agric Food Chem. 2014;62(51):12346-59. Published 2014 Dec 24. doi:10.1021/jf504469t
PMID: 25436390
Citation 4: Moore RW, Hargis BM, Porter TE, et al. Ovoinhibitor in the chicken bursa of Fabricius: identification, isolation, and localization. Cell Tissue Res. 2004;317(3):247-51. Published 2004 Sep. doi:10.1007/s00441-004-0910-x
PMID: 15252730
Citation 5: Bourin M, Gautron J, Berges M, et al. Antimicrobial potential of egg yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg. J Agric Food Chem. 2011;59(23):12368-74. Published 2011 Dec 14. doi:10.1021/jf203339t
PMID: 22010862
Citation 6: Kinoshita K, Shimogiri T, Okamoto S, et al. Linkage mapping of chicken ovoinhibitor and ovomucoid genes to chromosome 13. Anim Genet. 2004;35(4):356-8. Published 2004 Aug. doi:10.1111/j.1365-2052.2004.01159.x
PMID: 15265086
Citation 7: FEENEY RE, STEVENS FC, OSUGA DT, et al. The specificities of chicken ovomucoid and ovoinhibitor. J Biol Chem. 1963;238:1415-8. Published 1963 Apr. doi:
PMID: 13944692
Citation 8: Gertler A, Ben-Valid I, Ben-Valid I. Stoichiometry of interaction of chicken ovoinhibitor with pancreatic trypsin, chymotrypsin and elastase I. Eur J Biochem. 1980;110(2):571-7. Published 1980 Sep. doi:10.1111/j.1432-1033.1980.tb04900.x
PMID: 6904299
Citation 9: Matsuda T, Watanabe K, Nakamura R, et al. Ovomucoid and ovoinhibitor isolated from chicken egg white are immunologically cross-reactive. Biochem Biophys Res Commun. 1983;110(1):75-81. Published 1983 Jan 14. doi:10.1016/0006-291x(83)91262-7
PMID: 6838526
Citation 10: Yolken RH, Willoughby R, Wee SB, et al. Sialic acid glycoproteins inhibit in vitro and in vivo replication of rotaviruses. J Clin Invest. 1987;79(1):148-54. Published 1987 Jan. doi:10.1172/JCI112775
PMID: 3025257
Citation 11: Moss GW, Marshall J, Morabito M, et al. An evolutionarily conserved binding site for serine proteinase inhibitors in large conductance calcium-activated potassium channels. Biochemistry. 1996;35(50):16024-35. Published 1996 Dec 17. doi:10.1021/bi961452k
PMID: 8973172
Citation 12: Zhu Y, Wang M, Lin H, et al. Identification of estrogen-responsive genes in chick liver. Cell Tissue Res. 2001;305(3):357-63. Published 2001 Sep. doi:10.1007/s004410100414
PMID: 11572089
Citation 13: Begum S, Saito A, Xu X, et al. Improved functional properties of the ovoinhibitor by conjugating with galactomannan. Biosci Biotechnol Biochem. 2003;67(9):1897-902. Published 2003 Sep. doi:10.1271/bbb.67.1897
PMID: 14519973
Citation 14: Begum S, Saito A, Kato A, et al. Expression and characterization of chicken ovoinhibitor in Pichia pastoris. Nahrung. 2003;47(5):359-63. Published 2003 Oct. doi:10.1002/food.200390082
PMID: 14609095
Citation 15: Martos G, López-Fandiño R, Molina E, et al. Immunoreactivity of hen egg allergens: influence on in vitro gastrointestinal digestion of the presence of other egg white proteins and of egg yolk. Food Chem. 2013;136(2):775-81. Published 2013 Jan 15. doi:10.1016/j.foodchem.2012.07.106
PMID: 23122126
5.2 Protein Sequence Databases
UniProt: P10184
5.3 3D Structure Databases
No PDB Ids found
AlphaFoldDB: P10184
5.4 Nucleotide Sequence Databases
Sl. no. Accession(s) Access Link(s)
1. AADN05000072 GenBank || EMBL
2. M16141 GenBank || EMBL
3. M15962 GenBank || EMBL
4. M16127 GenBank || EMBL
5. M16128 GenBank || EMBL
6. M16129 GenBank || EMBL
7. M16130 GenBank || EMBL
8. M16131 GenBank || EMBL
9. M16132 GenBank || EMBL
10. M16133 GenBank || EMBL
11. M16134 GenBank || EMBL
12. M16135 GenBank || EMBL
13. M16136 GenBank || EMBL
14. M16137 GenBank || EMBL
15. M16138 GenBank || EMBL
16. M16139 GenBank || EMBL
17. M16140 GenBank || EMBL
18. AY225161 GenBank || EMBL
CCDS: Not found
RefSeq: NP_001025783.2
5.5 Protein-Protein Interaction Databases
STRING: 9031.ENSGALP00000005535
IntAct: P10184
MINT: Not found
DIP: Not found
BioGRID: Not found
5.6 Ligand Databases
BindingDB: Not found
DrugBank: Not found
ChEMBL: Not found
5.7 Family & Domain Databases
InterPro: IPR002350, IPR036058
PANTHER: Not found
PROSITE: PS00282, PS51465
5.8 Genome Annotation Databases
Ensembl: Not found
KEGG: Not found
5.9 Phylogenomic Databases
GeneTree: Not found
5.10 Enzyme & Pathway Databases
BRENDA: Not found
BioCyc: Not found
5.11 Protein-RNA Interaction Databases
RNAct: Not found




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