AMPDB_122 | Lysozyme C-2

1 General Description

AMPDB ID: AMPDB_122

Protein Names: Lysozyme C-2 (EC 3.2.1.17) (1;4-beta-N-acetylmuramidase C) (Lysozyme C type M)

Protein Family: Glycosyl hydrolase 22 family

Gene Name: Lyz2 Lyz Lyzs

Source Organism: Mus musculus (Mouse)

Protein Length: 148 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

MKTLLTLGLLLLSVTAQAKVYERCEFARTLKRNGMAGYYGVSLADWVCLAQHESNYNTRATNYNRGDQSTDYGIFQINSRYWCNDGKTPRAVNACGINCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRAHCQNRDLSQYIRNCGV

FASTA format

Counts of Amino Acids

'A': 16, 'R': 13, 'N': 11, 'D': 8, 'C': 8, 'Q': 9, 'E': 3, 'G': 10, 'H': 2, 'I': 7, 'L': 13, 'K': 5, 'M': 2, 'F': 2, 'P': 2, 'S': 7, 'T': 9, 'W': 4, 'Y': 8, 'V': 9

Frequencies of Amino Acids

'A': 10.81%, 'R': 8.78%, 'N': 7.43%, 'D': 5.41%, 'C': 5.41%, 'Q': 6.08%, 'E': 2.03%, 'G': 6.76%, 'H': 1.35%, 'I': 4.73%, 'L': 8.78%, 'K': 3.38%, 'M': 1.35%, 'F': 1.35%, 'P': 1.35%, 'S': 4.73%, 'T': 6.08%, 'W': 2.7%, 'Y': 5.41%, 'V': 6.08%

Missing Amino Acid(s)

No Amino Acid(s) are missing in this protein

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

F, H, M, P

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	16689 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	81.149	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	17.756	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.332	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.823	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	8.915	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	6.685	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.291, 0.203, 0.23 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.095	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	33920, 34420	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Gram Negative Bacteria and Gram Positive Bacteria

4.2 Antimicrobial Activity

Antimicrobial, Bacteriolytic enzyme, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

Glycosidase, Hydrolase

4.4 Inhibitory Effect

4.5 Other Biological Activity

Milk protein, Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Cross M, Mangelsdorf I, Wedel A, et al. Mouse lysozyme M gene: isolation, characterization, and expression studies. Proc Natl Acad Sci U S A. 1988;85(17):6232-6. Published 1988 Sep. doi:10.1073/pnas.85.17.6232

PMID: 3413093

Citation 2: Carninci P, Kasukawa T, Katayama S, et al. The transcriptional landscape of the mammalian genome. Science. 2005;309(5740):1559-63. Published 2005 Sep 2. doi:10.1126/science.1112014

PMID: 16141072

Citation 3: Gerhard DS, Wagner L, Feingold EA, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121-7. Published 2004 Oct. doi:10.1101/gr.2596504

PMID: 15489334

Citation 4: Ganz T, Gabayan V, Liao HI, et al. Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycan. Blood. 2003;101(6):2388-92. Published 2003 Mar 15. doi:10.1182/blood-2002-07-2319

PMID: 12411294

Citation 5: Markart P, Korfhagen TR, Weaver TE, et al. Mouse lysozyme M is important in pulmonary host defense against Klebsiella pneumoniae infection. Am J Respir Crit Care Med. 2004;169(4):454-8. Published 2004 Feb 15. doi:10.1164/rccm.200305-669OC

PMID: 14617511

Citation 6: Markart P, Faust N, Graf T, et al. Comparison of the microbicidal and muramidase activities of mouse lysozyme M and P. Biochem J. 2004;380(Pt 2):385-92. Published 2004 Jun 1. doi:10.1042/BJ20031810

PMID: 14977423

Citation 7: Sinha P, Chi HH, Kim HR, et al. Mouse lysozyme-M knockout mice reveal how the self-determinant hierarchy shapes the T cell repertoire against this circulating self antigen in wild-type mice. J Immunol. 2004;173(3):1763-71. Published 2004 Aug 1. doi:10.4049/jimmunol.173.3.1763

PMID: 15265906

Citation 8: Cole AM, Thapa DR, Gabayan V, et al. Decreased clearance of Pseudomonas aeruginosa from airways of mice deficient in lysozyme M. J Leukoc Biol. 2005;78(5):1081-5. Published 2005 Nov. doi:10.1189/jlb.0205073

PMID: 16204648

Citation 9: Huttlin EL, Jedrychowski MP, Elias JE, et al. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010;143(7):1174-89. Published 2010 Dec 23. doi:10.1016/j.cell.2010.12.001

PMID: 21183079

Citation 10: Obita T, Ueda T, Imoto T, et al. Solution structure and activity of mouse lysozyme M. Cell Mol Life Sci. 2003;60(1):176-84. Published 2003 Jan. doi:10.1007/s000180300012

PMID: 12613666

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: P08905

5.4 Nucleotide Sequence Databases

Sl. no.	Accession(s)	Access Link(s)
1.	M21050	GenBank \|\| EMBL
2.	M21047	GenBank \|\| EMBL
3.	M21048	GenBank \|\| EMBL
4.	M21049	GenBank \|\| EMBL
5.	AK148516	GenBank \|\| EMBL
6.	AK150998	GenBank \|\| EMBL
7.	AK153244	GenBank \|\| EMBL
8.	AK153475	GenBank \|\| EMBL
9.	AK159276	GenBank \|\| EMBL
10.	AK159640	GenBank \|\| EMBL
11.	BC002069	GenBank \|\| EMBL
12.	BC019611	GenBank \|\| EMBL
13.	BC054463	GenBank \|\| EMBL

CCDS: CCDS48694.1

RefSeq: NP_059068.1

5.5 Protein-Protein Interaction Databases

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

PROSITE: PS00128, PS51348

5.8 Genome Annotation Databases

Ensembl: ENSGT00940000153832

5.9 Phylogenomic Databases

GeneTree: ENSGT00940000153832

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

Download