AMPDB_1072 | Neutrophil antibiotic peptide NP-3B

PEPTIDE SUMMARY

Neutrophil antibiotic peptide NP-3B

1 General Description

AMPDB ID: AMPDB_1072

Protein Names: Neutrophil antibiotic peptide NP-3B (RatNP-3b) (Neutrophil defensin 3)

Protein Family: Alpha-defensin family

Gene Name: Nil

Source Organism: Rattus norvegicus (Rat)

Protein Length: 87 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

2.1 Sequence

MRTLILLTTLLLLALHTQAESPQGSTKEAPDEEQDISVFFGGDKGTALQDAAVKAGVTCSCRTSSCRFGERLSGACRLNGRIYRLCC

FASTA format

2.2 Composition

Counts of Amino Acids

'A': 8, 'R': 7, 'N': 1, 'D': 4, 'C': 6, 'Q': 4, 'E': 5, 'G': 8, 'H': 1, 'I': 3, 'L': 12, 'K': 3, 'M': 1, 'F': 3, 'P': 2, 'S': 7, 'T': 8, 'W': 0, 'Y': 1, 'V': 3

Frequencies of Amino Acids

'A': 9.2%, 'R': 8.05%, 'N': 1.15%, 'D': 4.6%, 'C': 6.9%, 'Q': 4.6%, 'E': 5.75%, 'G': 9.2%, 'H': 1.15%, 'I': 3.45%, 'L': 13.79%, 'K': 3.45%, 'M': 1.15%, 'F': 3.45%, 'P': 2.3%, 'S': 8.05%, 'T': 9.2%, 'W': 0%, 'Y': 1.15%, 'V': 3.45%

Missing Amino Acid(s)

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

H, M, N, Y

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	9352.74 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	86.437	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	40.889	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	-0.033	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.718	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	7.758	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	0.726	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.253, 0.207, 0.299 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.046	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	1490, 1865	Computed by ProtParam module (biopython 1.79)

4 Activity Details

4.1 Target Organism(s)

S.aureus (Gram-positive)

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Defensin, Fungicide, Anti-gram-Positive

4.3 Enzymatic Activity

Not found

4.4 Inhibitory Effect

Not found

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

5.1.1 PubMed

Citation 1: Eisenhauer PB, Harwig SS, Szklarek D, et al. Purification and antimicrobial properties of three defensins from rat neutrophils. Infect Immun. 1989;57(7):2021-7. Published 1989 Jul. doi:10.1128/iai.57.7.2021-2027.1989

PMID: 2543629

5.2 Protein Sequence Databases

UniProt: Q9Z1F1

5.3 3D Structure Databases

No PDB Ids found

AlphaFoldDB: Q9Z1F1

5.4 Nucleotide Sequence Databases