AMPDB_1014 | Thiocillin GE2270

1 General Description

AMPDB ID: AMPDB_1014

Protein Names: Thiocillin GE2270 (Antibiotic GE2270)

Protein Family: Thiocillin family

Gene Name: Nil

Source Organism: Planobispora rosea

Protein Length: 14 AA

Protein Existence: Evidence at protein level

2 Protein Sequence & Composition

SCNCVCGFCCSCSP

FASTA format

Counts of Amino Acids

'A': 0, 'R': 0, 'N': 1, 'D': 0, 'C': 6, 'Q': 0, 'E': 0, 'G': 1, 'H': 0, 'I': 0, 'L': 0, 'K': 0, 'M': 0, 'F': 1, 'P': 1, 'S': 3, 'T': 0, 'W': 0, 'Y': 0, 'V': 1

Frequencies of Amino Acids

'A': 0%, 'R': 0%, 'N': 7.14%, 'D': 0%, 'C': 42.86%, 'Q': 0%, 'E': 0%, 'G': 7.14%, 'H': 0%, 'I': 0%, 'L': 0%, 'K': 0%, 'M': 0%, 'F': 7.14%, 'P': 7.14%, 'S': 21.43%, 'T': 0%, 'W': 0%, 'Y': 0%, 'V': 7.14%

Missing Amino Acid(s)

A, D, E, H, I, K, L, M, Q, R, T, W, Y

Most Occurring Amino Acid(s)

Less Occurring Amino Acid(s)

F, G, N, P, V

Hydrophobic Amino Acid(s) Count

Hydrophilic Amino Acid(s) Count

Basic Amino Acid(s) Count

Acidic Amino Acid(s) Count

Modified Amino Acid(s) Count

Modified Amino Acid(s) Frequencies

Computed by biopython (version 1.79) & proteinAnalysis (version 1)

3 Physicochemical Properties

Sl. No.	Properties	Values	Reference
1.	Molecular Mass	1412.66 Da	Computed by ProtParam module (biopython 1.79)
2.	Aliphatic Index	20.714	Computed by ProtParam module (biopython 1.79)
3.	Instability Index (Half Life)	79.8	Computed by ProtParam module (biopython 1.79)
4.	Hydrophobicity (GRAVY)	1.007	Computed by ProtParam module (biopython 1.79)
5.	Hydrophobic Moment	0.227	Computed by ProtParam module (biopython 1.79)
6.	Isoelectric Point	5.632	Computed by ProtParam module (biopython 1.79)
7.	Charge (at pH 7)	-0.374	Computed by ProtParam module (biopython 1.79)
8.	Secondary Structure Fraction	0.143, 0.429, 0 [Helix, Turn, Sheet]	Computed by ProtParam module (biopython 1.79)
9.	Aromaticity	0.071	Computed by ProtParam module (biopython 1.79)
10.	Molar Extinction Coefficient (cysteine\|cystine)	0, 375	Computed by ProtParam module (biopython 1.79)

4.1 Target Organism(s)

Gram Negative Bacteria and Gram Positive Bacteria

4.2 Antimicrobial Activity

Antibiotic, Antimicrobial, Bacteriocin, Anti-gram-negative, Anti-gram-Positive

4.3 Enzymatic Activity

4.4 Inhibitory Effect

4.5 Other Biological Activity

Non-hemolytic, Non-ribosomal

Activity data manually curated from Literature and UniProt

5 Database Cross-references

5.1 Literature Database

Citation 1: Selva E, Beretta G, Montanini N, et al. Antibiotic GE2270 a: a novel inhibitor of bacterial protein synthesis. I. Isolation and characterization. J Antibiot (Tokyo). 1991;44(7):693-701. Published 1991 Jul. doi:10.7164/antibiotics.44.693

PMID: 1908853

Citation 2: Colombo L, Stella S, Selva E, et al. Contribution of mass spectrometry to the structural confirmation of components of the antibiotic GE2270 complex. Rapid Commun Mass Spectrom. 1995;9(8):717-22. Published 1995. doi:10.1002/rcm.1290090817

PMID: 7647369

Citation 3: Parmeggiani A, Nissen P, Nissen P. Elongation factor Tu-targeted antibiotics: four different structures, two mechanisms of action. FEBS Lett. 2006;580(19):4576-81. Published 2006 Aug 21. doi:10.1016/j.febslet.2006.07.039

PMID: 16876786

Citation 4: MÃ¼ller HM, Delgado O, Bach T, et al. Total synthesis of the thiazolyl peptide GE2270 A. Angew Chem Int Ed Engl. 2007;46(25):4771-4. Published 2007. doi:10.1002/anie.200700684

PMID: 17503407

Citation 5: Kettenring J, Colombo L, Ferrari P, et al. Antibiotic GE2270 a: a novel inhibitor of bacterial protein synthesis. II. Structure elucidation. J Antibiot (Tokyo). 1991;44(7):702-15. Published 1991 Jul. doi:10.7164/antibiotics.44.702

PMID: 1880060

Citation 6: Tavecchia P, Gentili P, Kurz M, et al. Revised structure of the antibiotic GE 2270A. J Antibiot (Tokyo). 1994;47(12):1564-7. Published 1994 Dec. doi:10.7164/antibiotics.47.1564

PMID: 7844053

Citation 7: Selva E, Ferrari P, Kurz M, et al. Components of the GE2270 complex produced by Planobispora rosea ATCC 53773. J Antibiot (Tokyo). 1995;48(9):1039-42. Published 1995 Sep. doi:10.7164/antibiotics.48.1039

PMID: 7592050

Citation 8: Heffron SE, Jurnak F, Jurnak F. Structure of an EF-Tu complex with a thiazolyl peptide antibiotic determined at 2.35 A resolution: atomic basis for GE2270A inhibition of EF-Tu. Biochemistry. 2000;39(1):37-45. Published 2000 Jan 11. doi:10.1021/bi9913597

PMID: 10625477

Citation 9: Parmeggiani A, Krab IM, Okamura S, et al. Structural basis of the action of pulvomycin and GE2270 A on elongation factor Tu. Biochemistry. 2006;45(22):6846-57. Published 2006 Jun 6. doi:10.1021/bi0525122

PMID: 16734421

5.2 Protein Sequence Databases

5.3 3D Structure Databases

	Sl. no.	PDB ID	Method	Resolution	Access Links	3D View

AlphaFoldDB: Q7M0J8

5.4 Nucleotide Sequence Databases

No entries found in GenBank or EMBL

RefSeq: Not found

5.5 Protein-Protein Interaction Databases

STRING: Not found

IntAct: Not found

BioGRID: Not found

5.6 Ligand Databases

BindingDB: Not found

DrugBank: Not found

ChEMBL: Not found

5.7 Family & Domain Databases

InterPro: Not found

PANTHER: Not found

PROSITE: Not found

5.8 Genome Annotation Databases

Ensembl: Not found

5.9 Phylogenomic Databases

GeneTree: Not found

5.10 Enzyme & Pathway Databases

BRENDA: Not found

BioCyc: Not found

5.11 Protein-RNA Interaction Databases

RNAct: Not found

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